Recombinant humaan Aldo-ketoreductase 1A1/AKR1A1-eiwit

Alcohol dehydrogenase [NADP+] (AKR1A1) is a member of the aldo/keto reductase superfamily and catalyzes the NADPH-dependent reduction of a variety of aromatic and aliphatic aldehydes to their corresponding alcohols. This protein is closely related (65% identity) to aldose reductase, an enzyme involved in the pathogenesis of some diabetic and galactosemic complications. It plays a role in the activation of procarcinogens, such as polycyclic aromatic hydrocarbon trans-dihydrodiols, and in the metabolism of various xenobiotics and drugs, including the anthracyclines doxorubicin (DOX) and daunorubicin (DAuN) . Recombinant human Alcohol dehydrogenase was expressed in E. coli and purified by using conventional chromatography techniques.

Dihydrodiol dehydrogenase 3, DD3, ARM, ALR, ALDR1, aldo-keto reductase family 1 member A1 (aldehyde reductase), Aldehyde reductase, Alcohol dehydrogenase [NADP+], Alcohol dehydrogenase, AKR1A1

E.coli

Human

Non-Tagged

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

36.5 kDa (325aa) confirmed by MALDI-TOF

Dihydrodiol dehydrogenase 3, DD3, ARM, ALR, ALDR1, aldo-keto reductase family 1 member A1 (aldehyde reductase), Aldehyde reductase, Alcohol dehydrogenase [NADP+], Alcohol dehydrogenase, AKR1A1, ATGP0433-10 µg, ATGP0433-20 µg, ATGP0433-50 µg, ATGP0433-100 µg, ATGP0433-250 µg, ATGP0433-500 µg, ATGP0433-1 mg, ATGP0433-010, ATGP0433-020, ATGP0433-050, ATGP0433-100, ATGP0433-250, ATGP0433-500, ATGP0433-01M

Bohren KM., et al. (1989) J Biol Chem. 264 (16) : 9547-51.; ; Vander Jaqt DL., et al. (1990) J Biol Chem. 265 (19) :10912-8.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 50 mM NaCl, 10% glycerol

Redox Proteins

NP_006057

P14550

Human

MAASCVLLHT GQKMPLIGLG TWKSEPGQVK AAVKYALSVG YRHIDCAAIY GNEPEIGEAL KEDVGPGKAV PREELFVTSK LWNTKHHPED VEPALRKTLA DLQLEYLDLY LMHWPYAFER GDNPFPKNAD GTICYDSTHY KETWKALEAL VAKGLVQALG LSNFNSRQID DILSVASVRP AVLQVECHPY LAQNELIAHC QARGLEVTAY SPLGSSDRAW RDPDEPVLLE EPVVLALAEKYGRSPAQILL RWQVQRKVIC IPKSITPSRI LQNIKVFDFT FSPEEMKQLN ALNKNWRYIV PMLTVDGKRV PRDAGHPLYP FNDPY