Recombinant humaan Aldo-ketoreductase 1B1/AKR1B1-eiwit

Aldose reductase (AKR1B1) is a member of the aldo-keto reductase (AKR) superfamily and catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. This protein is implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Recombinant Aldose reductase (AKR1B) protein was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is >800 pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction of 1.0 pmole DL-glyceraldehyde in the presence of NADPH per minute at pH7.0 at 37C.

Aldo-keto reductase family1 member B1, AKR1B1, ADR, ALDR1, ALR2, AR, Aldose reductase, AKR1B 1, Aldehyde reductase, aldr 1, aldr-1, Lii5-2 CTCL tumor antigen, low Km aldose reductase

E.coli

Human

Non-Tagged

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Specific activity is >800pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction of 1.0pmole DL-glyceraldehyde in the presence of NADPH per minute at pH7.0 at 37C.

35.8 kDa (316aa) confirmed by MALDI-TOF

Aldose reductase, Aldo-keto reductase family1 member B1, AKR1B1, ADR, ALDR1, ALR2, AR, Aldo-keto reductase family1 member B1, Aldose reductase, Aldo-keto reductase family1, member B1 AKR1B 1, Aldehyde reductase, aldehyde reductase 1, aldo-keto reductase family 1, member B1 aldr 1, aldr-1, Lii5-2 CTCL tumor antigen, low Km aldose reductase., ALR0901-10 µg, ALR0901-20 µg, ALR0901-50 µg, ALR0901-100 µg, ALR0901-250 µg, ALR0901-500 µg, ALR0901-1 mg, ALR0901-10, ALR0901-20, ALR0901-50, ALR0901-100, ALR0901-250, ALR0901-500, ALR0901-1

Ruiz FX., et al: (2009) Chem Biol Interact.178 (1-3) ; ; Donaqhue KC., et al: Diabet Med. 22 (10) :1315-20;

Fatmawati S, et al. Ganoderic acid Df, a new triterpenoid with aldose reductase inhibitory activity from the fruiting body of Ganoderma lucidum. (Fitoterapia. 2010) {https://pubmed.ncbi.nlm.nih.gov/20603199/}; ; Onoda T, et al. Inhibitory activities of selected Kampo formulations on human aldose reductase. (BMC Complement Altern Med. 2014) {https://pubmed.ncbi.nlm.nih.gov/25374323/}; ; Kuroda M, et al. Chemical Constituents of the Leaves of Tussilago farfara and their Aldose Reductase Inhibitory Activity. (Nat Prod Commun. 2016) {https://pubmed.ncbi.nlm.nih.gov/30475501/}; ; Ishibashi F, et al. Synthesis and structure-activity relationship study of aldose reductase inhibiting marine alkaloid lukianol A and its derivatives. (Biosci Biotechnol Biochem. 2022) {https://pubmed.ncbi.nlm.nih.gov/36441013/}; ; Matsuo Y, et al. Lanceolanone A, a new biflavanone, and a chalcone glucoside from the flower heads of Coreopsis lanceolata and their aldose reductase inhibitory activity and AMPK activation. (J Nat Med. 2023) {https://pubmed.ncbi.nlm.nih.gov/36068394/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Redox Proteins

NP_001619.1

P15121

Human

MASRLLLNNG AKMPILGLGT WKSPPGQVTE AVKVAIDVGY RHIDCAHVYQ NENEVGVAIQ EKLREQVVKR EELFIVSKLW CTYHEKGLVK GACQKTLSDL KLDYLDLYLI HWPTGFKPGK EFFPLDESGN VVPSDTNILD TWAAMEELVD EGLVKAIGIS NFNHLQVEMI LNKPGLKYKP AVNQIECHPY LTQEKLIQYC QSKGIVVTAY SPLGSPDRPW AKPEDPSLLE DPRIKAIAAK HNKTTAQVLI RFPMQRNLVV IPKSVTPERI AENFKVFDFE LSSQDMTTLL SYNRNWRVCA LLSCTSHKDY PFHEEF