Recombinant humaan Aldo-ketoreductase 1D1/AKR1D1-eiwit

Aldo-keto reductase family 1, member D1, also known as AKR1D1, is a member of the AKR superfamily. The AKR family of proteins are soluble NADPH oxidoreductases. They play important roles in the metabolism of drugs, carcinogens and reactive aldehydes. AKR1D1 is responsible for the catalysis of the 5-beta-reduction of bile acid intermediates and steroid hormones which carry a delta (4) -3-one structure. AKR1D1 is highly expressed in liver, colon and testis. Deficiency of this enzyme may contribute to hepatic dysfunction. Recombinant human AKR1D1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Aldo-keto reductase family 1 member D1, SRD5B1, Delta 4-3-ketosteroid-5-beta-reductase

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

39.5 kDa (346aa) confirmed by MALDI-TOF

Aldo-keto reductase1D1, AKR1D1, Aldo-keto reductase family 1 member D1, Aldo-keto reductase family 1, member D1, 3o5bred, CBAS2, SRD5B1, ATGP0953-10 µg, ATGP0953-20 µg, ATGP0953-50 µg, ATGP0953-100 µg, ATGP0953-250 µg, ATGP0953-500 µg, ATGP0953-1 mg, ATGP0953-10, ATGP0953-20, ATGP0953-50, ATGP0953-100, ATGP0953-250, ATGP0953-500, ATGP0953-1

Charbonneau A., et al. (2001) Biochim Biophys Acta. 1517:228-235.; ; Lemonde H A., et al. (2003) Gut. 52:1494-1499.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 100 mM NaCl

Metabolism

NP_005980.1

P51857

Human

MDLSAASHRI PLSDGNSIPI IGLGTYSEPK STPKGACATS VKVAIDTGYR HIDGAYIYQN EHEVGEAIRE KIAEGKVRRE DIFYCGKLWA TNHVPEMVRP TLERTLRVLQ LDYVDLYIIE VPMAFKPGDE IYPRDENGKW LYHKSNLCAT WEAMEACKDA GLVKSLGVSN FNRRQLELIL NKPGLKHKPV SNQVECHPYF TQPKLLKFCQ QHDIVITAYS PLGTSRNPIW VNVSSPPLLK DALLNSLGKR YNKTAAQIVL RFNIQRGVVV IPKSFNLERI KENFQIFDFS LTEEEMKDIE ALNKNVRFVE LLMWRDHPEY PFHDEY