Recombinant humaan Aldo-ketoreductase 1D1/AKR1D1-eiwit

AKR1D1 also known as 3-oxo-5beta-steroid 4-deghydrogenase isoform 1, is a member of the AKR superfamily. The AKR family of proteins are soluble NADPH oxidoreductases. They play important roles in the metabolism of drugs, carcinogens and reactive aldehydes. AKR1D1 is responsible for the catalysis of the 5-betareduction of bile acid intermediates and steroid hormones which carry a delta (4) -3-one structure. AKR1D1 is highly expressed in liver, colon and testis. Deficiency of this enzyme may contribute to hepatic dysfunction. Recombinant AKR1D1 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Aldo-keto reductase family 1 member D1, SRD5B1, Delta 4-3-ketosteroid-5-beta-reductase

E.coli

Human

Non-Tagged

SDS-PAGE

1 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

37.3 kDa (326aa)

Aldo-keto reductase1D1, AKR1D1, 3o5bred, CBAS2, SRD5B1, ATGP3292-10 µg, ATGP3292-20 µg, ATGP3292-50 µg, ATGP3292-100 µg, ATGP3292-250 µg, ATGP3292-500 µg, ATGP3292-1 mg, ATGP3292-10, ATGP3292-20, ATGP3292-50, ATGP3292-100, ATGP3292-250, ATGP3292-500, ATGP3292-1

Moore S A., et al. (1995) J Mol Biol. 249 (1) :195-214; ; Gould S J., et al. (1988) Anal Biochem. 175 (1) :5-13

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 1mM DTT, 0.1M NaCl, 10% glycerol

Metabolism

NP_005980

P51857

Human

MDLSAASHRI PLSDGNSIPI IGLGTYSEPK STPKGACATS VKVAIDTGYR HIDGAYIYQN EHEVGEAIRE KIAEGKVRRE DIFYCGKLWA TNHVPEMVRP TLERTLRVLQ LDYVDLYIIE VPMAFKPGDE IYPRDENGKW LYHKSNLCAT WEAMEACKDA GLVKSLGVSN FNRRQLELIL NKPGLKHKPV SNQVECHPYF TQPKLLKFCQ QHDIVITAYS PLGTSRNPIW VNVSSPPLLK DALLNSLGKR YNKTAAQIVL RFNIQRGVVV IPKSFNLERI KENFQIFDFS LTEEEMKDIE ALNKNVRFVE LLMWRDHPEY PFHDEY