Recombinant humaan Aldo-ketoreductase 7A2/AKR7A2-eiwit

Aldo-keto reductases, such as AKR7A2, are involved in the detoxification of aldehydes and ketones. This protein can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the nonbinding AFB1 dialcohol. It may be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen. Also, it has been proposed previously to catalyze the NADPH-dependent reduction of succinic semialdehyde (SSA) to gamma-Hydroxybutyrate in human brain. Recombinant AKR7A2 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 1000 pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction 1.0 pmole of 1,2-Naphthoquinone presence of NADPH per minute at pH 7.0 at 25C.

Aldo-keto reductase family 7 member A2, Aflatoxin B1 aldehyde reductase member 2, AFB1 aldehyde reductase 1 (AFB1-AR 1), Aldoketoreductase 7, Succinic semialdehyde reductase (SSA reductase), AFAR, AFAR1, AKR7

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

Specific activity is > 1000pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction 1.0pmole of 1,2-Naphthoquinone presence of NADPH per minute at pH 7.0 at 25C.

44 kDa (398aa) confirmed by MALDI-TOF

Aflatoxin B1 aldehyde reductase member2, AKR7A2, aldo-keto reductase family 7 member A2, Aflatoxin B1 aldehyde reductase member 2, AFAR, AFAR1, AFB1-AR1, AKR7, aldo-keto reductase family 7, member A2 AFB1 AR1, AFB1AR1, aflatoxin aldehyde reductase, aflatoxin beta1 aldehyde reductase, Aiar, aldo keto reductase family 7, aldo keto reductase family 7, member A2, aldoketoreductase 7., ATGP0478-10 µg, ATGP0478-20 µg, ATGP0478-50 µg, ATGP0478-100 µg, ATGP0478-250 µg, ATGP0478-500 µg, ATGP0478-1 mg, ATGP0478-10, ATGP0478-20, ATGP0478-50, ATGP0478-100, ATGP0478-250, ATGP0478-500, ATGP0478-1

Bodreddigari S., et al. (2008) Chem Res Toxicol. 21 (5) :1134-42.; ; Malaspina P., et al. (2009) Hum Genomics. 3 (2) :106-20.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol

Redox Proteins

NP_003680

O43488

Human

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSELEM LSAASRVVSR AAVHCALRSP PPEARALAMS RPPPPRVASV LGTMEMGRRM DAPASAAAVR AFLERGHTEL DTAFMYSDGQ SETILGGLGL GLGGGDCRVK IATKANPWDG KSLKPDSVRS QLETSLKRLQ CPQVDLFYLH APDHGTPVEE TLHACQRLHQ EGKFVELGLS NYASWEVAEI CTLCKSNGWI LPTVYQGMYN ATTRQVETEL FPCLRHFGLR FYAYNPLAGG LLTGKYKYED KDGKQPVGRF FGNSWAETYR NRFWKEHHFE AIALVEKALQ AAYGASAPSV TSAALRWMYH HSQLQGAHGD AVILGMSSLE QLEQNLAATE EGPLEPAVVD AFNQAWHLVA HECPNYFR