Recombinant humaan Aldo-ketoreductase 7A3/AKR7A3-eiwit

Aldo-keto reductase family 7, member A 3, also known as AKR7A3, is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member includes a number of related monomeric NADPH-dependent oxidoreductases, such as aldehyde reductase, aldose reductase, prostaglandin F synthase, xylose reductase, rho crystallin, and many others. AKR7A3 is involved in the detoxification of aldehydes and ketones. The activity of AKR7A3 may detoxify the aflatoxin B1 (AFB1) dialdehyde, which reacts with proteins, and thereby inhibits AFB 1 induced toxicity. Recombinant human AKR7A3, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 700 pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction 1.0 pmole of 1,2-Naphthoquinone presence of NADPH per minute at pH 7.0 at 25C.

Aldo-keto reductase family 7 member A 3, Aflatoxin B1 aldehyde reductase member 3, AFB1 aldehyde reductase 2 (AFAR2), AFB1 AR 2, Aflatoxin aldehyde reductase, Aflatoxin B1 aldehyde reductase 2

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

Specific activity is > 700pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction 1.0pmole of 1,2-Naphthoquinone presence of NADPH per minute at pH 7.0 at 25C.

41.6 kDa (370aa) confirmed by MALDI-TOF

Aflatoxin B1 aldehyde reductase member3, AKR7A3, Aldo-keto reductase family 7 member A 3, Aflatoxin B1 aldehyde reductase member 3, AFB1 aldehyde reductase 2 (AFAR2), Aldo-keto reductase family 7, member A 3 AFAR 2, AFAR2, AFB1 AR 2, Aflatoxin aldehyde reductase, Aflatoxin B1 aldehyde reductase 2, Aflatoxin B1 aldehyde reductase member 3, Aldo keto reductase family 7 member A3, OTTHuMP00000002623., ATGP0476-10 µg, ATGP0476-20 µg, ATGP0476-50 µg, ATGP0476-100 µg, ATGP0476-250 µg, ATGP0476-500 µg, ATGP0476-1 mg, ATGP0476-10, ATGP0476-20, ATGP0476-50, ATGP0476-100, ATGP0476-250, ATGP0476-500, ATGP0476-1

Kniqht LP., et al. (1999) Carcinogenesis. 20 (7) :1215-23. ; ; Bodreddiqari S., et al. (2008) Chem Res Toxicol. 21 (5) :1134-42.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Redox Proteins

AAH25709.1

O95154

Human

M SRQLSRARPA TVLGAMEMGR RMDAPTSAAV TRAFLERGHT EIDTAFVYSE GQSETILGGL GLRLGGSDCR VKIDTKAIPL FGNSLKPDSL RFQLETSLKR LQCPRVDLFY LHMPDHSTPV EETLRACHQL HQEGKFVELG LSNYAAWEVA EICTLCKSNG WILPTVYQGM YNAITRQVET ELFPCLRHFG LRFYAFNPLA GGLLTGKYKY EDKDGKQPVG RFFGNTWAEM YRNRYWKEHH FEGIALVEKA LQAAYGASAP SMTSATLRWM YHHSQLQGAH GDAVILGMSS LEQLEQNLAA AEEGPLEPAV VDAFNQAWHL VAHECPNYFR