Recombinant humaan Aldo-ketoreductase 7A3/AKR7A3-eiwit

Aldo-keto reductase family 7, member A 3, also known as AKR7A3, is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member includes a number of related monomeric NADPH-dependent oxidoreductases, such as aldehyde reductase, aldose reductase, prostaglandin F synthase, xylose reductase, rho crystallin, and many others. AKR7A3 is involved in the detoxification of aldehydes and ketones. The activity of AKR7A3 may detoxify the aflatoxin B1 (AFB1) dialdehyde, which reacts with proteins, and thereby inhibits AFB 1 induced toxicity. Recombinant human AKR7A3 was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 800 pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction 1.0 pmole of 1,2-Naphthoquinone presence of NADPH per minute at pH 7.0 at 25C.

Aldo-keto reductase family 7 member A 3, Aflatoxin B1 aldehyde reductase member 3, AFB1 aldehyde reductase 2 (AFAR2), AFB1 AR 2, Aflatoxin aldehyde reductase, Aflatoxin B1 aldehyde reductase 2

E.coli

Human

Non-Tagged

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Specific activity is > 800pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction 1.0pmole of 1,2-Naphthoquinone presence of NADPH per minute at pH 7.0 at 25C.

37.7 kDa (331aa)

AFB1 aldehyde reductase2, AKR7A3, Aldo-keto reductase family 7, member A 3, Aflatoxin B1 aldehyde reductase member 3, AFB1 aldehyde reductase 2 (AFAR2), ATGP3286-10 µg, ATGP3286-20 µg, ATGP3286-50 µg, ATGP3286-100 µg, ATGP3286-250 µg, ATGP3286-500 µg, ATGP3286-1 mg, ATGP3286-10, ATGP3286-20, ATGP3286-50, ATGP3286-100, ATGP3286-250, ATGP3286-500, ATGP3286-1

Ahmed MM., et al. (2011) Hepatology. 54 (4) :1322-32. ; ; Bodreddiqari S., et al. (2008) Chem Res Toxicol. 21 (5) :1134-42.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 10% glycerol

Metabolism

AAH25709

O95154

Human

MSRQLSRARP ATVLGAMEMG RRMDAPTSAA VTRAFLERGH TEIDTAFVYS EGQSETILGG LGLRLGGSDC RVKIDTKAIP LFGNSLKPDS LRFQLETSLK RLQCPRVDLF YLHMPDHSTP VEETLRACHQ LHQEGKFVEL GLSNYAAWEV AEICTLCKSN GWILPTVYQG MYNAITRQVE TELFPCLRHF GLRFYAFNPL AGGLLTGKYK YEDKDGKQPV GRFFGNTWAE MYRNRYWKEH HFEGIALVEK ALQAAYGASA PSMTSATLRW MYHHSQLQGA HGDAVILGMS SLEQLEQNLA AAEEGPLEPA VVDAFNQAWH LVAHECPNYF R