Recombinant humaan ALDOB-eiwit

Fructose-bisphosphate aldolase B, also known as ALDOB, is one of three known aldolase isoenzymes, and is found in kidney and small adult intestine where it is associated with aldolases A or C. ALDOB catalyzes the reversible cleavage of fructose 1-phosphate into dihydroxyacetone phosphate and glyceraldehyde. It is regulated by the hormones Insulin and glucagon and has been implicated in hereditary fructose intolerance disease. Recombinant human ALDOB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Fructose-bisphosphate aldolase B, ALDB, ALDO2, Fructose bisphosphate aldolase B

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

42 kDa (388aa) confirmed by MALDI-TOF

ALDOB, Fructose-bisphosphate aldolase B, ALDB, ALDO2, Fructose bisphosphate aldolase B, ATGP1404-10 µg, ATGP1404-20 µg, ATGP1404-50 µg, ATGP1404-100 µg, ATGP1404-250 µg, ATGP1404-500 µg, ATGP1404-1 mg, ATGP1404-10, ATGP1404-20, ATGP1404-50, ATGP1404-100, ATGP1404-250, ATGP1404-500, ATGP1404-1

Caffe A R., et al. (1994) J Comp Neurol. 348:291-297.; ; Dehnes Y., et al. (1998) J Neurosci. 18:3606-3619.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Metabolism

NP_000026.2

P05062

Human

MAHRFP ALTQEQKKEL SEIAQSIVAN GKGILAADES VGTMGNRLQR IKVENTEENR RQFREILFSV DSSINQSIGG VILFHETLYQ KDSQGKLFRN ILKEKGIVVG IKLDQGGAPL AGTNKETTIQ GLDGLSERCA QYKKDGVDFG KWRAVLRIAD QCPSSLAIQE NANALARYAS ICQQNGLVPI VEPEVIPDGD HDLEHCQYVT EKVLAAVYKA LNDHHVYLEG TLLKPNMVTA GHACTKKYTP EQVAMATVTA LHRTVPAAVP GICFLSGGMS EEDATLNLNA INLCPLPKPW KLSFSYGRAL QASALAAWGG KAANKEATQE AFMKRAMANC QAAKGQYVHT GSSGAASTQS LFTACYTY