Recombinant humaan ALDOC-eiwit

ALDOC, also known as aldolase C, is a member of the class I fructose-biphosphate aldolase family. This protein is a glycolytic enzyme that catalyzes the reversible aldol cleavage of fructose-1, 6-biphosphate and fructose 1-phosphate to dihydroxyacetone phosphate and either glyceraldehyde-3-phosphate or glyceraldehydes respectively. It is expressed specifically in the hippocampus and Purkinje cells of the brain. Recombinant human ALDOC protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Aldolase C fructose-bisphosphate., Aldolase C, fructose-bisphosphate., ALDC

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

41.6 kDa (384aa) confirmed by MALDI-TOF

ALDOC, Aldolase C fructose-bisphosphate., Aldolase C, fructose-bisphosphate., ALDC, ATGP0980-10 µg, ATGP0980-20 µg, ATGP0980-50 µg, ATGP0980-100 µg, ATGP0980-250 µg, ATGP0980-500 µg, ATGP0980-1 mg, ATGP0980-10, ATGP0980-20, ATGP0980-50, ATGP0980-100, ATGP0980-250, ATGP0980-500, ATGP0980-1

Wang CF., et al. (2007) Anim Genet. 38 (3) :203-10. ; ; Arakaki TL., et al. (2004) Protein Sci. 13 (12) :3077-84.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.

Cancer

NP_005156

P09972

Human

MPHSYPALSA EQKKELSDIA LRIVAPGKGI LAADESVGSM AKRLSQIGVE NTEENRRLYR QVLFSADDRV KKCIGGVIFF HETLYQKDDN GVPFVRTIQD KGIVVGIKVD KGVVPLAGTD GETTTQGLDG LSERCAQYKK DGADFAKWRC VLKISERTPS ALAILENANV LARYASICQQ NGIVPIVEPE ILPDGDHDLK RCQYVTEKVL AAVYKALSDH HVYLEGTLLK PNMVTPGHAC PIKYTPEEIA MATVTALRRT VPPAVPGVTF LSGGQSEEEA SFNLNAINRC PLPRPWALTF SYGRALQASA LNAWRGQRDN AGAATEEFIK RAEVNGLAAQ GKYEGSGEDG GAAAQSLYIA NHAY