Recombinant humaan ALDOC-eiwit

ALDOC, also as known as fructose bisphosphate C, is a member of the class 1 fructose-biphosphate aldolase family. This protein is a ubiquitous enzyme that catalyzes the reversible aldol cleavage of fructose-bisphosphate and fructose 1-phospate to digydroxyacetone phosphate and either glyceral-dehyde-3-phosphate or glyceraldegyde, respectively. It is expressed specifically in the hippocampus and Purkinje cells of the brain. Recombinant human ALDOC was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 6 units/mg, one unit will convert 1.0 umol of fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehydes 3- phosphate per minute at pH 7.5 at 37C

Fructose bisphosphate aldolase C, ALDC

E.coli

Human

Non-Tagged

Enzyme Activity, SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

Specific activity is > 6unit/mg, one unit will convert 1.0 umol of fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehydes 3- phosphate per minute at pH 7.5 at 37C

39.4 kDa (364aa) confirmed by MALDI-TOF

ALDOC, Fructose bisphosphate aldolase C, ALDC, ATGP3709-10 µg, ATGP3709-20 µg, ATGP3709-50 µg, ATGP3709-100 µg, ATGP3709-250 µg, ATGP3709-500 µg, ATGP3709-1 mg, ATGP3709-10, ATGP3709-20, ATGP3709-50, ATGP3709-100, ATGP3709-250, ATGP3709-500, ATGP3709-1

Caspi M., et al. (2014) Mol Cancer. 13:164.; ; Arakaki TL., et al. (2004) Protein Sci. 13 (12) : 3077-84

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 2mM DTT, 0.1M NaCl

Cancer

NP_005156

P09972

Human

MPHSYPALSA EQKKELSDIA LRIVAPGKGI LAADESVGSM AKRLSQIGVE NTEENRRLYR QVLFSADDRV KKCIGGVIFF HETLYQKDDN GVPFVRTIQD KGIVVGIKVD KGVVPLAGTD GETTTQGLDG LSERCAQYKK DGADFAKWRC VLKISERTPS ALAILENANV LARYASICQQ NGIVPIVEPE ILPDGDHDLK RCQYVTEKVL AAVYKALSDH HVYLEGTLLK PNMVTPGHAC PIKYTPEEIA MATVTALRRT VPPAVPGVTF LSGGQSEEEA SFNLNAINRC PLPRPWALTF SYGRALQASA LNAWRGQRDN AGAATEEFIK RAEVNGLAAQ GKYEGSGEDG GAAAQSLYIA NHAY