Recombinant humaan alfa-Galactosidase A/GLA-eiwit

GLA, also known as alpha-galactosidase A, is homodimeric glycoprotein that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. It can catalyze the hydrolysis of melibiose into galactose and glucose. A variety of mutations in this gene affect the synthesis, processing, and stability of this enzyme, which causes Fabry disease, a rare lysosomal storage disorder that results from a failure to catabolize alpha-D-galactosyl glycolipid moieties. Recombinant human GLA, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

GLA, GALA

Baculovirus

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

46.4 kDa (406aa)

A-Galactosidase A, GLA, GLA, GALA, ATGP3225-10 µg, ATGP3225-20 µg, ATGP3225-50 µg, ATGP3225-100 µg, ATGP3225-250 µg, ATGP3225-500 µg, ATGP3225-1 mg, ATGP3225-10, ATGP3225-20, ATGP3225-50, ATGP3225-100, ATGP3225-250, ATGP3225-500, ATGP3225-1

Garman S.C., et al. (2004) J. Mol. Biol. 337:319-335.; ; Sakuraba H., et al. (1990) Am. J. Hum. Genet. 47:784-789.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Cardiovascular

NP_000160

P06280

Human