Recombinant humaan alfa-glad Muscle ActiCTA2-eiwit

ACTA2 belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. Recombinant human ACTA2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Aortic smooth muscle actin, Alpha-actin-2, Cell growth-inhibiting gene 46 protein, Actin, Aortic smooth muscle, Intermediate form, ACTSA, ACTVS, GIG46

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

44.4 kDa (400aa) confirmed by MALDI-TOF

ACTA2, Actin aortic smooth muscle, Actin, aortic smooth muscle, AAT6, ACTSA, MYMY5, ATGP1719-10 µg, ATGP1719-20 µg, ATGP1719-50 µg, ATGP1719-100 µg, ATGP1719-250 µg, ATGP1719-500 µg, ATGP1719-1 mg, ATGP1719-10, ATGP1719-20, ATGP1719-50, ATGP1719-100, ATGP1719-250, ATGP1719-500, ATGP1719-1

Sakai, H., et al. (2012) Hum. Genet. 131 (4), 591-599; ; Regalado, E., et al. (2011) Am. J. Med. Genet. A 155A (9), 2125-2130.

Gordon O, et al. α-actinin accounts for the bioactivity of actin preparations in inducing STAT target genes in Drosophila melanogaster. (Elife. 2018) {https://pubmed.ncbi.nlm.nih.gov/30260317/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol

Cardiovascular

NP_001604.1

P62736

Human

EEEDS TALVCDNGSG LCKAGFAGDD APRAVFPSIV GRPRHQGVMV GMGQKDSYVG DEAQSKRGIL TLKYPIEHGI ITNWDDMEKI WHHSFYNELR VAPEEHPTLL TEAPLNPKAN REKMTQIMFE TFNVPAMYVA IQAVLSLYAS GRTTGIVLDS GDGVTHNVPI YEGYALPHAI MRLDLAGRDL TDYLMKILTE RGYSFVTTAE REIVRDIKEK LCYVALDFEN EMATAASSSS LEKSYELPDG QVITIGNERF RCPETLFQPS FIGMESAGIH ETTYNSIMKC DIDIRKDLYA NNVLSGGTTM YPGIADRMQK EITALAPSTM KIKIIAPPER KYSVWIGGSI LASLSTFQQM WISKQEYDEA GPSIVHRKCF