Recombinant humaan alfa-L-Fucosidase 1/FUCA1-eiwit

FUCA1, also known as tissue alpha-L-fucosidase, is responsible for hydrolyzing the alpha-1, 6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins. Fucosidosis is an autosomal recessive lysosomal storage disease resulting from absence of alpha-L-fucosidase activity. Recombinant human FUCA1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Tissue alpha-L-fucosidase, α-L-fucosidase 1, Tissue alpha-L-fucosidase, a-L-fucosidase 1, Alpha-L-fucosidase I, Alpha-L-fucoside fucohydrolase 1, Alpha-L-fucosidase 1

Baculovirus

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

51.7 kDa (445aa)

Tissue a-L-Fucosidase, FUCA1, Tissue alpha-L-fucosidase, ATGP3170-10 µg, ATGP3170-20 µg, ATGP3170-50 µg, ATGP3170-100 µg, ATGP3170-250 µg, ATGP3170-500 µg, ATGP3170-1 mg, ATGP3170-10, ATGP3170-20, ATGP3170-50, ATGP3170-100, ATGP3170-250, ATGP3170-500, ATGP3170-1

Yang M. et al., (1993) Biochim. Biophys. Acta. 1182 (3) :245-249.; ; Seo H.-C. et al., (1994) Hum. Mol. Genet. 3 (11) :2065-2066.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Signal Transduction

NP_000138

P04066

Human

VRRAQPPRRY TPDWPSLDSR PLPAWFDEAK FGVFIHWGVF SVPAWGSEWF WWHWQGEGRP QYQRFMRDNY PPGFSYADFG PQFTARFFHP EEWADLFQAA GAKYVVLTTK HHEGFTNWPS PVSWNWNSKD VGPHRDLVGE LGTALRKRNI RYGLYHSLLE WFHPLYLLDK KNGFKTQHFV SAKTMPELYD LVNSYKPDLI WSDGEWECPD TYWNSTNFLS WLYNDSPVKD EVVVNDRWGQ NCSCHHGGYY NCEDKFKPQS LPDHKWEMCT SIDKFSWGYR RDMALSDVTE ESEIISELVQ TVSLGGNYLL NIGPTKDGLI VPIFQERLLA VGKWLSINGE AIYASKPWRV QWEKNTTSVW YTSKGSAVYA IFLHWPENGV LNLESPITTS TTKITMLGIQ GDLKWSTDPD KGLFISLPQL PPSAVPAEFA WTIKLTGVKH HHHHH