Recombinant humaan alfa-L-Fucosidase 2/FUCA2-eiwit

FuCA2 is a plasma alpha-L-fucosidase, which represents 10-20% of the total cellular fucosidase activity. This protein is a member of the glycosyl hydrolase 29 family, and catalyzes the hydrolysis of the alpha-1, 6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins. FuCA2 is essential for Helicobacter pylori adhesion to human gastric cancer cells. Recombinant human FuCA2 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Alpha-L-fucosidase 2, Alpha-L-fucoside fucohydrolase 2, α-L-fucosidase 2, plasma fucosidase, a-L-fucosidase 2

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

53.3 kDa (462aa)

FuCA2, Plasma alpha-L-fucosidase, Plasma alpha-L-fucosidase, Fucosidase, alpha-L- 2, plasma, dJ20N2.5, RP1-20N2.5, ATGP2902-10 µg, ATGP2902-20 µg, ATGP2902-50 µg, ATGP2902-100 µg, ATGP2902-250 µg, ATGP2902-500 µg, ATGP2902-1 mg, ATGP2902-10, ATGP2902-20, ATGP2902-50, ATGP2902-100, ATGP2902-250, ATGP2902-500, ATGP2902-1

Clark H.F., et al. (2003) Genome Re., 13:2265-2270

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM phosphate (pH 8.0) containing 10% glycerol

Metabolism

NP_114409

Q9BTY2

Human

MGSSHHHHHH SSGLVPRGSH MGSHSATRFD PTWESLDARQ LPAWFDQAKF GIFIHWGVFS VPSFGSEWFW WYWQKEKIPK YVEFMKDNYP PSFKYEDFGP LFTAKFFNAN QWADIFQASG AKYIVLTSKH HEGFTLWGSE YSWNWNAIDE GPKRDIVKEL EVAIRNRTDL RFGLYYSLFE WFHPLFLEDE SSSFHKRQFP VSKTLPELYE LVNNYQPEVL WSDGDGGAPD QYWNSTGFLA WLYNESPVRG TVVTNDRWGA GSICKHGGFY TCSDRYNPGH LLPHKWENCM TIDKLSWGYR REAGISDYLT IEELVKQLVE TVSCGGNLLM NIGPTLDGTI SVVFEERLRQ MGSWLKVNGE AIYETHTWRS QNDTVTPDVW YTSKPKEKLV YAIFLKWPTS GQLFLGHPKA ILGATEVKLL GHGQPLNWIS LEQNGIMVEL PQLTIHQMPC KWGWALALTN VI