Recombinant humaan ALKBH2-eiwit

Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2, also known ALKBH2, belongs to the ALKB family. Expressed in heart, colon, liver, testis, ovary, prostate and small intestine, ALKBH2 uses iron as a cofactor and functions as a dioxygenase that catalyzes the repair of alkylated DNA and RNA containing 1-methyladenine and 3-meth-ylcytosine. ALKBH2 is functionally activated by ascorbate and requires oxygen and alpha-ketoglutarate for enzymatic activity. Recombinant human ALKBH2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2, ABH2, Oxy DC1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

31.4 kDa (281aa) confirmed by MALDI-TOF

ALKBH2, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2, ABH2, Oxy DC1, ATGP1016-10 µg, ATGP1016-20 µg, ATGP1016-50 µg, ATGP1016-100 µg, ATGP1016-250 µg, ATGP1016-500 µg, ATGP1016-1 mg, ATGP1016-10, ATGP1016-20, ATGP1016-50, ATGP1016-100, ATGP1016-250, ATGP1016-500, ATGP1016-1

Rinqvoll J., et al. (2008) Cancer Res. 68:4142-4149.; ; Lee D H., et al. (2005) J Biol Chem. 280:39448-39459.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol

Enzymes & Proteases

NP_001138847.1

Q6NS38

Human

MDRFLVKGAQ GGLLRKQEEQ EPTGEEPAVL GGDKESTRKR PRREAPGNGG HSAGPSWRHI RAEGLDCSYT VLFGKAEADE IFQELEKEVE YFTGALARVQ VFGKWHSVPR KQATYGDAGL TYTFSGLTLS PKPWIPVLER IRDHVSGVTG QTFNFVLINR YKDGCDHIGE HRDDERELAP GSPIASVSFG ACRDFVFRHK DSRGKSPSRR VAVVRLPLAH GSLLMMNHPT NTHWYHSLPV RKKVLAPRVN LTFRKILLTK K