Recombinant humaan ALKBH3-eiwit

ALKBH3 is a member of the ALKB family of proteins and functions as a dioxygenase with a preference for RNA and single stranded DNA substrates. This protein repairs 1-methyladenine and 3-methylcytosine lesions in alkylated DNA and RNA and its activity is stimulated by ascorbate. ALKBH3 is expressed in a wide variety of tissues and localizes to the cytoplasm and the nucleus. Recombinant human ALKBH3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3, ABH3, DEPC-1, DEPC1, PCA1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

37.8 kDa (325aa) confirmed by MALDI-TOF

ALKBH3, ATGP0981-10 µg, ATGP0981-20 µg, ATGP0981-50 µg, ATGP0981-100 µg, ATGP0981-250 µg, ATGP0981-500 µg, ATGP0981-1 mg, ATGP0981-10, ATGP0981-20, ATGP0981-50, ATGP0981-100, ATGP0981-250, ATGP0981-500, ATGP0981-1

Ougland R., et al. (2004) Mol Cell. 16 (1) :107-16. ; ; Duncan T., et al. (2002) Proc Natl Acad Sci u S A. 99 (26) :16660-5.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.

Enzymes & Proteases

NP_631917.1

Q96Q83

Human

M EEKRRRARVQ GAWAAPVKSQ AIAQPATTAK SHLHQKPGQT WKNKEHHLSD REFVFKEPQQ VVRRAPEPRV IDREGVYEIS LSPTGVSRVC LYPGFVDVKE ADWILEQLCQ DVPWKQRTGI REDITYQQPR LTAWYGELPY TYSRITMEPN PHWHPVLRTL KNRIEENTGH TFNSLLCNLY RNEKDSVDWH SDDEPSLGRC PIIASLSFGA TRTFEMRKKP PPEENGDYTY VERVKIPLDH GTLLIMEGAT QADWQHRVPK EYHSREPRVN LTFRTVYPDP RGAPW