Recombinant humaan ALT1/GPT1-eiwit

GPT, also known as alanine aminotransferases (ALT1), catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. This protein plays a key role in the intermediary metabolism of glucose and amino acids. It is widely used as an index of liver integrity or hepatocellular damage in clinical settings. Recombinant human GPT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Specific activity is > 100units/mg, and is defined as the amount of enzyme that cleaves 1umole of L-Alanine to L-Glutamate per minute at pH 7.5 at 37C

Alanine aminotransferase 1, AAT1, ALT1, GPT1

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

Specific activity is > 100unit/mg, and is defined as the amount of enzyme that cleaves 1umole of L-Alanine to L-Glutamate per minute at pH 7.5 at 37C

56.8 kDa (516aa) confirmed by MALDI-TOF

ALT1, GPT1, alanine aminotransferase 1, AAT1, ALT1, GPT1, ATGP3176-10 µg, ATGP3176-20 µg, ATGP3176-50 µg, ATGP3176-100 µg, ATGP3176-250 µg, ATGP3176-500 µg, ATGP3176-1 mg, ATGP3176-10, ATGP3176-20, ATGP3176-50, ATGP3176-100, ATGP3176-250, ATGP3176-500, ATGP3176-1

Sohocki M.M. et al. (1997) Genomics 40: 247-252.; ; Matthews C.C. et al. (2003) Brain Res. 978: 59-64.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 2mM DTT

Metabolism

NP_005300.1

P24298

Human

MASSTGDRSQ AVRHGLRAKV LTLDGMNPRV RRVEYAVRGP IVQRALELEQ ELRQGVKKPF TEVIRANIGD AQAMGQRPIT FLRQVLALCV NPDLLSSPNF PDDAKKRAER ILQACGGHSL GAYSVSSGIQ LIREDVARYI ERRDGGIPAD PNNVFLSTGA SDAIVTVLKL LVAGEGHTRT GVLIPIPQYP LYSATLAELG AVQVDYYLDE ERAWALDVAE LHRALGQARD HCRPRALCVI NPGNPTGQVQ TRECIEAVIR FAFEERLFLL ADEVYQDNVY AAGSQFHSFK KVLMEMGPPY AGQQELASFH STSKGYMGEC GFRGGYVEVV NMDAAVQQQM LKLMSVRLCP PVPGQALLDL VVSPPAPTDP SFAQFQAEKQ AVLAELAAKA KLTEQVFNEA PGISCNPVQG AMYSFPRVQL PPRAVERAQE LGLAPDMFFC LRLLEETGIC VVPGSGFGQR EGTYHFRMTI LPPLEKLRLL LEKLSRFHAK FTLEYS