Recombinant humaan ALT2/GPT2-eiwit

Alanine aminotransferase 2, also known as GPT2, catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Subsequently, they play a key role in the intermediary metabolism of glucose and amino acids. GPT2 expression is high in muscle, fat and kidney. Recombinant human GPT2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alanine aminotransferase 2, Glutamate pyruvate transaminase 2, GPT 2, Glutamic--alanine transaminase 2, Glutamic--pyruvic transaminase 2, AAT2, ALT2

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

60.3 kDa (546aa)

AAT 2, AAT2, Alanine aminotransferase 2, ALT 2, ALT2, Glutamate pyruvate transaminase 2, Glutamic alanine transaminase 2, Glutamic-alanine transaminase 2, Glutamic--alanine transaminase 2, Glutamic-pyruvic transaminase 2, Glutamic--pyruvic transaminase 2, GPT 2, GPT2, Glutamic pyruvic transaminase 2, ATGP1914-10 µg, ATGP1914-20 µg, ATGP1914-50 µg, ATGP1914-100 µg, ATGP1914-250 µg, ATGP1914-500 µg, ATGP1914-1 mg, ATGP1914-010, ATGP1914-020, ATGP1914-050, ATGP1914-100, ATGP1914-250, ATGP1914-500, ATGP1914-01M

Sohocki M M., et al. (1997) Genomics. 40:247-252; ; Matthews C C., et al. (2003) Hepatology. 39: 1297-1302.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 30% glycerol 0.2M NaCl, 2mM DTT

Metabolism

NP_597700.1

Q8TD30

Human

MQRAAAL VRRGCGPRTP SSWGRSQSSA AAEASAVLKV RPERSRRERI LTLESMNPQV KAVEYAVRGP IVLKAGEIEL ELQRGIKKPF TEVIRANIGD AQAMGQQPIT FLRQVMALCT YPNLLDSPSF PEDAKKRARR ILQACGGNSL GSYSASQGVN CIREDVAAYI TRRDGGVPAD PDNIYLTTGA SDGISTILKI LVSGGGKSRT GVMIPIPQYP LYSAVISELD AIQVNYYLDE ENCWALNVNE LRRAVQEAKD HCDPKVLCII NPGNPTGQVQ SRKCIEDVIH FAWEEKLFLL ADEVYQDNVY SPDCRFHSFK KVLYEMGPEY SSNVELASFH STSKGYMGEC GYRGGYMEVI NLHPEIKGQL VKLLSVRLCP PVSGQAAMDI VVNPPVAGEE SFEQFSREKE SVLGNLAKKA KLTEDLFNQV PGIHCNPLQG AMYAFPRIFI PAKAVEAAQA HQMAPDMFYC MKLLEETGIC VVPGSGFGQR EGTYHFRMTI LPPVEKLKTV LQKVKDFHIN FLEKYA