Recombinant humaan AMELX-eiwit

AMELX is a member of the amelogenin family of extracellular matrix proteins. Amelogenins are involved in biomineralization during tooth enamel development. Mutations in AMELX cause X-linked amelogenesis imperfecta. Alternative splicing results in multiple transcript variants encoding different isoforms. Recombinant human AMELX protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

22.3 kDa (198aa) confirmed by MALDI-TOF

AMELX, ATGP1859-10 µg, ATGP1859-20 µg, ATGP1859-50 µg, ATGP1859-100 µg, ATGP1859-250 µg, ATGP1859-500 µg, ATGP1859-1 mg, ATGP1859-10, ATGP1859-20, ATGP1859-50, ATGP1859-100, ATGP1859-250, ATGP1859-500, ATGP1859-1

Patir A, Seymen F, et al. (2008) . Caries Res. 42 (5) :394-400.; ; Zhu L, Tanimoto K, et al. (2009) . Cells Tissues Organs. 189 (1-4) :203-6.;

Terada C, et al. Effect of Amelogenin Coating of a Nano-Modified Titanium Surface on Bioactivity. Int J Mol Sci. (2018 Apr) {https://pubmed.ncbi.nlm.nih.gov/29695118/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 0.2M NaCl, 30% glycerol, 1mM DTT

Signal Transduction

NP_001133

Q99217

Human

MPLPPHP GHPGYINFSY EVLTPLKWYQ SIRPPYPSYG YEPMGGWLHH QIIPVLSQQH PPTHTLQPHH HIPVVPAQQP VIPQQPMMPV PGQHSMTPIQ HHQPNLPPPA QQPYQPQPVQ PQPHQPMQPQ PPVHPMQPLP PQPPLPPMFP MQPLPPMLPD LTLEAWPSTD KTKREEVD