Recombinant humaan amnionloos eiwit

AMN, also known as amnionless, is a type I transmembrane protein. A complex of amnionless and cubilin forms the cubam receptor. This protein is necessary for efficient absorption of vitamin B12. It is thought to modulate bone morphogenetic protein (BMP) receptor function by serving as an accessory or coreceptor, and thus facilitates or hinders BMP binding. Recombinant human AMN protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Protein amnionless, PRO1028

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

38.2 kDa (361aa) confirmed by MALDI-TOF

Amnionless, protein amnionless, PRO1028, ATGP1866-10 µg, ATGP1866-20 µg, ATGP1866-50 µg, ATGP1866-100 µg, ATGP1866-250 µg, ATGP1866-500 µg, ATGP1866-1 mg, ATGP1866-10, ATGP1866-20, ATGP1866-50, ATGP1866-100, ATGP1866-250, ATGP1866-500, ATGP1866-1

Fyfe J.C., et al. (2004) Blood 103:1573-1579; ; Tomihara-Newberger, C., et al. (1999) Dev. Biol. 204: 34-54.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol,1mM DTT

Metabolism

NP_112205

Q9BXJ7

Human

VSKLWVP NTDFDVAANW SQNRTPCAGG AVEFPADKMV SVLVQEGHAV SDMLLPLDGE LVLASGAGFG VSDVGSHLDC GAGEPAVFRD SDRFSWHDPH LWRSGDEAPG LFFVDAERVP CRHDDVFFPP SASFRVGLGP GASPVRVRSI SALGRTFTRD EDLAVFLASR AGRLRFHGPG ALSVGPEDCA DPSGCVCGNA EAQPWICAAL LQPLGGRCPQ AACHSALRPQ GQCCDLCGAV VLLTHGPAFD LERYRARILD TFLGLPQYHG LQVAVSKVPR SSRLREADTE IQVVLVENGP ETGGAGRLAR ALLADVAENG EALGVLEATM RESGAHVWGS S