Recombinant humaan AMPK alfa-1-eiwit

PRKAA1 also known as 5-AMP-activated protein kinase catalytic subunit alpha-1 isoform 1. PRKAA1 is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK) . AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinasen activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Recombinant human PRKAA1 protein, fused to His-tag at N-terminus, was expressed in E. coli.

PRKAA1, 5-AMP-activated protein kinase catalytic subunit alpha-1 isoform 1, AMPK, AMPKa11

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

34.3 kDa (302aa)

AMPK a1, 5-AMP-activated protein kinase catalytic subunit alpha-1 isoform 1, AMPK, AMPKa11, ATGP2985-10 µg, ATGP2985-20 µg, ATGP2985-50 µg, ATGP2985-100 µg, ATGP2985-250 µg, ATGP2985-500 µg, ATGP2985-1 mg, ATGP2985-10, ATGP2985-20, ATGP2985-50, ATGP2985-100, ATGP2985-250, ATGP2985-500, ATGP2985-1

Imamura K., et al. (2001) Biochem. Biophys. Res. Commun. 287:562-567; ; Yang W., et al. (2001) J. Biol. Chem. 276:38341-38344

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Neuroscience

NP_006242.2

Q13131

Human

MRRLSSW RKMATAEKQK HDGRVKIGHY ILGDTLGVGT FGKVKVGKHE LTGHKVAVKI LNRQKIRSLD VVGKIRREIQ NLKLFRHPHI IKLYQVISTP SDIFMVMEYV SGGELFDYIC KNGRLDEKES RRLFQQILSG VDYCHRHMVV HRDLKPENVL LDAHMNAKIA DFGLSNMMSD GEFLRTSCGS PNYAAPEVIS GRLYAGPEVD IWSSGVILYA LLCGTLPFDD DHVPTLFKKI CDGIFYTPQY LNPSVISLLK HMLQVDPMKR ATIKDIREHE WF