Recombinant humaan AMPK beta 1/PRKAB1-eiwit

5'-AMP-activated protein kinase subunit beta-1, also known as PRKAB1, inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3) . Recombinant human PRKAB1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

5'-AMP-activated protein kinase subunit beta-1, AMPK, HAMPKb, PRKAB1, Protein kinase AMP-activated non-catalytic subunit beta 1, AMPK subunit beta-1, AMPKb

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

32.8 kDa (293aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

AMPK b1, 5'-AMP-activated protein kinase subunit beta-1, AMPK, HAMPKb, ATGP1786-10 µg, ATGP1786-20 µg, ATGP1786-50 µg, ATGP1786-100 µg, ATGP1786-250 µg, ATGP1786-500 µg, ATGP1786-1 mg, ATGP1786-10, ATGP1786-20, ATGP1786-50, ATGP1786-100, ATGP1786-250, ATGP1786-500, ATGP1786-1

Hardie D.G., et al. (2007) Nat. Rev. Mol. Cell Biol. 8:774-785; ; Towler M.C., et al. (2007) Circ. Res. 100:328-341;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Metabolism

NP_006244.2

Q9Y478

Human

MGNTSSE RAALERHGGH KTPRRDSSGG TKDGDRPKIL MDSPEDADLF HSEEIKAPEK EEFLAWQHDL EVNDKAPAQA RPTVFRWTGG GKEVYLSGSF NNWSKLPLTR SHNNFVAILD LPEGEHQYKF FVDGQWTHDP SEPIVTSQLG TVNNIIQVKK TDFEVFDALM VDSQKCSDVS ELSSSPPGPY HQEPYVCKPE ERFRAPPILP PHLLQVILNK DTGISCDPAL LPEPNHVMLN HLYALSIKDG VMVLSATHRY KKKYVTTLLY KPI