Recombinant humaan AMPK gamma1/PRKAG1-eiwit

5'-AMP-activated protein kinase subunit gamma-1 isoform 1, also known as PRKAG1, is a regulatory subunit of the AMP-activated protein kinase (AMPK) . AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed. Recombinant human PRKAG1 protein, fused to His-tag at N-terminus, was expressed in E. coli.

5'-AMP-activated protein kinase subunit gamma-1 isoform 1, AMPKG, Protein kinase AMP-activated non-catalytic subunit gamma 1, AMPK subunit gamma-1

E.coli

Human

His-Tag

SDS-PAGE, Denatured

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

40 kDa (354aa)

PRKAG1, 5'-AMP-activated protein kinase subunit gamma-1 isoform 1, AMPKG, ATGP2334-10 µg, ATGP2334-20 µg, ATGP2334-50 µg, ATGP2334-100 µg, ATGP2334-250 µg, ATGP2334-500 µg, ATGP2334-1 mg, ATGP2334-10, ATGP2334-20, ATGP2334-50, ATGP2334-100, ATGP2334-250, ATGP2334-500, ATGP2334-1

Zaha VG., et al. (2012) Circ Res. 111 (6) :800-14; ; Za tara G., et al. (2008) Biochem Pharmacol. 76 (10) :1263-75.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Signal Transduction

NP_002724

P54619

Human

METVISS DSSPAVENEH PQETPESNNS VYTSFMKSHR CYDLIPTSSK LVVFDTSLQV KKAFFALVTN GVRAAPLWDS KKQSFVGMLT ITDFINILHR YYKSALVQIY ELEEHKIETW REVYLQDSFK PLVCISPNAS LFDAVSSLIR NKIHRLPVID PESGNTLYIL THKRILKFLK LFITEFPKPE FMSKSLEELQ IGTYANIAMV RTTTPVYVAL GIFVQHRVSA LPVVDEKGRV VDIYSKFDVI NLAAEKTYNN LDVSVTKALQ HRSHYFEGVL KCYLHETLET IINRLVEAEV HRLVVVDEND VVKGIVSLSD ILQALVLTGG EKKP