Recombinant humaan AMSH-LP eiwit

STAMBPL1 is a zinc metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains, but doesn't cleave 'Lys-48'-linked polyubiquitin chains. The JAMM motif is essential for the protease activity. Recombinant human STAMBPL1 protein, fused to His-tag at N-terminus was expressed in E. coli and purified by using conventional chromatography techniques.

AMSH-like protease, AMSH-like protease, ALMalpha, AMSH-FP, AMSH-LP, bA399O19.2

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

52.1 kDa (458aa)

AMSH-LP, AMSH-like protease, AMSH-like protease, ALMalpha, AMSH-FP, AMSH-LP, bA399O19.2, ATGP1955-10 µg, ATGP1955-20 µg, ATGP1955-50 µg, ATGP1955-100 µg, ATGP1955-250 µg, ATGP1955-500 µg, ATGP1955-1 mg, ATGP1955-10, ATGP1955-20, ATGP1955-50, ATGP1955-100, ATGP1955-250, ATGP1955-500, ATGP1955-1

Nakamura M, Tanaka N, et al. (2006) . Genes Cells. 11 (6) :593-606.; ; Kikuchi K, Ishii N, et al. (2003) . Biochem Biophys Res Commun. 306 (3) :637-43.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 20% glycerol, 0.1mM PMSF

Epigenomics (Transcription & Translation)

NP_065850

Q96FJ0

Human

MDQPFTVN SLKKLAAMPD HTDVSLSPEE RVRALSKLGC NITISEDITP RRYFRSGVEM ERMASVYLEE GNLENAFVLY NKFITLFVEK LPNHRDYQQC AVPEKQDIMK KLKEIAFPRT DELKNDLLKK YNVEYQEYLQ SKNKYKAEIL KKLEHQRLIE AERKRIAQMR QQQLESEQFL FFEDQLKKQE LARGQMRSQQ TSGLSEQIDG SALSCFSTHQ NNSLLNVFAD QPNKSDATNY ASHSPPVNRA LTPAATLSAV QNLVVEGLRC VVLPEDLCHK FLQLAESNTV RGIETCGILC GKLTHNEFTI THVIVPKQSA GPDYCDMENV EELFNVQDQH DLLTLGWIHT HPTQTAFLSS VDLHTHCSYQ LMLPEAIAIV CSPKHKDTGI FRLTNAGMLE VSACKKKGFH PHTKEPRLFS ICKHVLVKDI KIIVLDLR