Recombinant humaan AMT-eiwit

AMT also known as Aminomethyltransferase, mitochondrial isoform 1. AMT is a component of the glycine cleavage system termed T-protein, reversibly catalyzes the degradation of the aminomethyl moiety of glycine attached to the lipoate cofactor of H-protein, resulting in the production of ammonia, 5, 10-methylenetetrahydrofolate, and dihydrolipoate-bearing H-protein in the presence of tetrahydrofolate. Recombinant human AMT was expressed in E. coli and purified by using conventional chromatography techniques

Aminomethyltransferase mitochondrial isoform 1, Aminomethyltransferase, mitochondrial isoform 1, GCE, GCST, GCVT, NKH

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

43.3 kDa (398aa) confirmed by MALDI-TOF

AMT, Aminomethyltransferase mitochondrial isoform 1, Aminomethyltransferase, mitochondrial isoform 1, GCE, GCST, GCVT, NKH, ATGP3051-10 µg, ATGP3051-20 µg, ATGP3051-50 µg, ATGP3051-100 µg, ATGP3051-250 µg, ATGP3051-500 µg, ATGP3051-1 mg, ATGP3051-10, ATGP3051-20, ATGP3051-50, ATGP3051-100, ATGP3051-250, ATGP3051-500, ATGP3051-1

Narisawa A., et al. (2012) Hum. Mol. Genet. 21 (7), 1496-1503; ; Kure S., et al. (2006) Hum. Mutat. 27 (4), 343-352;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 30% glycerol, 1mM DTT

Neuroscience

NP_000472

P48728

Human

AQEVLRR TPLYDFHLAH GGKMVAFAGW SLPVQYRDSH TDSHLHTRQH CSLFDVSHML QTKILGSDRV KLMESLVVGD IAELRPNQGT LSLFTNEAGG ILDDLIVTNT SEGHLYVVSN AGCWEKDLAL MQDKVRELQN QGRDVGLEVL DNALLALQGP TAAQVLQAGV ADDLRKLPFM TSAVMEVFGV SGCRVTRCGY TGEDGVEISV PVAGAVHLAT AILKNPEVKL AGLAARDSLR LEAGLCLYGN DIDEHTTPVE GSLSWTLGKR RRAAMDFPGA KVIVPQLKGR VQRRRVGLMC EGAPMRAHSP ILNMEGTKIG TVTSGCPSPS LKKNVAMGYV PCEYSRPGTM LLVEVRRKQQ MAVVSKMPFV PTNYYTLK