Recombinant humaan AMTN-eiwit

AMTN belongs to the amelotin family. It could be a cell adhesion protein involved in the maturation of tooth enamel. AMTN is a recently discovered secreted enamel protein predominantly expressed during the maturation stage of enamel formation. It accumulates in a basal lamina-like structure at the interface between ameloblasts and enamel mineral and it co-localizes with another recently described enamel protein, odontogenic ameloblast-associated protein (ODAM) . Recombinant human AMTN protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Amelotin, uNQ689

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

22.2 kDa (216aa) confirmed by MALDI-TOF

AMTN, ATGP1829-10 µg, ATGP1829-20 µg, ATGP1829-50 µg, ATGP1829-100 µg, ATGP1829-250 µg, ATGP1829-500 µg, ATGP1829-1 mg, ATGP1829-10, ATGP1829-20, ATGP1829-50, ATGP1829-100, ATGP1829-250, ATGP1829-500, ATGP1829-1

Holcroft J, et al. (2011) Eur J Oral Sci. Dec;119 Suppl 1:301-6.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 20% glycerol, 2mM DTT

Signal Transduction

NP_997722

Q6UX39

Human

LPQLKPA LGLPPTKLAP DQGTLPNQQQ SNQVFPSLSL IPLTQMLTLG PDLHLLNPAA GMTPGTQTHP LTLGGLNVQQ QLHPHVLPIF VTQLGAQGTI LSSEELPQIF TSLIIHSLFP GGILPTSQAG ANPDVQDGSL PAGGAGVNPA TQGTPAGRLP TPSGTDDDFA VTTPAGIQRS THAIEEATTE SANGIQ