Recombinant humaan ANAPC13-eiwit

ANAPC13, also known as anaphase-promoting complex subunit 13, is component of the anaphase promoting complex, a large ubiquitin-protein ligase that controls cell cycle progression by regulating the degradation of cell cycle regulators such as B-type cyclins. This protein is evolutionarily conserved and is required for the integrity and ubiquitin ligase activity of the anaphase promoting complex Recombinant human ANAPC13 protein fused to T7-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Anaphase-promoting complex subunit 13, APC13, SWM1

E.coli

Human

T7-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

10 kDa (89aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

ANAPC13, Anaphase-promoting complex subunit 13, APC13, SWM1, ATGP1409-10 µg, ATGP1409-20 µg, ATGP1409-50 µg, ATGP1409-100 µg, ATGP1409-250 µg, ATGP1409-500 µg, ATGP1409-1 mg, ATGP1409-10, ATGP1409-20, ATGP1409-50, ATGP1409-100, ATGP1409-250, ATGP1409-500, ATGP1409-1

Schwickart M., et al. (2004) Mol. Cell. Biol. 24:3562-3576; ; Jin L., et al. (2008) Cell. 133:653-665

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT, 0.1M NaCl

Ubiquitination

NP_056206

Q9BS18

Human

MASMTGGQQM GRGSHMDSEV QRDGRILDLI DDAWREDKLP YEDVAIPLNE LPEPEQDNGG TTESVKEQEM KWTDLALQYL HENVPPIGN