Recombinant humaan Angiopoëtine 2/ANGPT2-eiwit

Angiopoietin-2, also known as ANGPT2, is a member of the ANG family. It has been identified as ligands for vascular endothelial-specific Tie2 receptor tyrosine kinase that interact with ANG2. This protein may be important growth factors in the generation of new blood vessels. Also, it is over-expression disrupts blood vessel formation in the mouse embryo. This protein is strongly expressed only at sites of vascular remodeling. It has been suggested that it may act synergistically with other cytokines such as vascular endothelial growth factor to promote tumor-associated Angiogenesis and tumor progression. Recombinant human Angiopoietin-2 fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Human Angiopoietin-2 is coated at 5 µg/mL (100 ul/well) can bind Human TIE-2 (CAT# ATGP3947) in a Functional ELISA assay.

Angiopoietin-2 isoform a, ANGPT2, AGPT2, ANG2

CHO Cell

Human

His-Tag

SDS-PAGE, Bioactivity

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Measured by its binding ability in a functional ELISA with Human TIE-2 (CAT# ATGP3947)

55.7 kDa (484aa)

Angiopoietin2, ATGP3853-10 µg, ATGP3853-20 µg, ATGP3853-50 µg, ATGP3853-100 µg, ATGP3853-250 µg, ATGP3853-500 µg, ATGP3853-1 mg, ATGP3853-10, ATGP3853-20, ATGP3853-50, ATGP3853-100, ATGP3853-250, ATGP3853-500, ATGP3853-1

Maisonpierre PC., et al. (1997) Science. 277:55-60.; ; Nowicki M., et al. (2017) Ann Hematol. 96:2103-2112.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Angiogenesis

NP_001138

O15123

Human

Tyr19