Recombinant humaan ANKRA2-eiwit

ANKRA2 contains 5 ANK repeats. This protein may facilitate endocytosis by linking megalin to components of the cytoskeleton or endocytic machinery. Recombinant human ANKRA2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ankyrin repeat family A protein 2, C11, C11-RNP3, hRPC11, My010, RPC10, RPC11, RPC12.5

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

36.7 kDa (336aa) confirmed by MALDI-TOF

ANKRA2, Ankyrin repeat family A protein 2, C11, C11-RNP3, hRPC11, My010, RPC10, RPC11, RPC12.5, ATGP2787-10 µg, ATGP2787-20 µg, ATGP2787-50 µg, ATGP2787-100 µg, ATGP2787-250 µg, ATGP2787-500 µg, ATGP2787-1 mg, ATGP2787-10, ATGP2787-20, ATGP2787-50, ATGP2787-100, ATGP2787-250, ATGP2787-500, ATGP2787-1

Wang, A.H., et al. (2005) J. Biol. Chem. 280 (32), 29117-29127; ; Rader, K., et al. (2000) J. Am. Soc. Nephrol. 11 (12), 2167-2178

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM DTT

Other

NP_075526

Q9H9E1

Human

MDTSTNL DIGAQLIVEE CPSTYSLTGM PDIKIEHPLD PNSEEGSAQG VAMGMKFILP NRFDMNVCSR FVKSLNEEDS KNIQDQVNSD LEVASVLFKA ECNIHTSPSP GIQVRHVYTP STTKHFSPIK QSTTLTNKHR GNEVSTTPLL ANSLSVHQLA AQGEMLYLAT RIEQENVINH TDEEGFTPLM WAAAHGQIAV VEFLLQNGAD PQLLGKGRES ALSLACSKGY TDIVKMLLDC GVDVNEYDWN GGTPLLYAVH GNHVKCVKML LESGADPTIE TDSGYNSMDL AVALGYRSVQ QVIESHLLKL LQNIKE