Recombinant humaan ANKRD1-eiwit

Ankyrin repeat domain-containing protein 1, also known as ANKRD1, may play an important role in endothelial cell activation. This protein may act as a nuclear transcription factor that negatively regulates the expression of cardiac genes. Recombinant human ANKRD1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ankyrin repeat domain-containing protein 1, ALRP, bA320F15.2, C-193, CARP, CVARP, MCARP

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

38.6 kDa (342aa) confirmed by MALDI-TOF

ANKRD1, Ankyrin repeat domain-containing protein 1, ALRP, bA320F15.2, C-193, CARP, CVARP, MCARP, ATGP1718-10 µg, ATGP1718-20 µg, ATGP1718-50 µg, ATGP1718-100 µg, ATGP1718-250 µg, ATGP1718-500 µg, ATGP1718-1 mg, ATGP1718-10, ATGP1718-20, ATGP1718-50, ATGP1718-100, ATGP1718-250, ATGP1718-500, ATGP1718-1

Chu W., et al. (1995) J. Biol. Chem. J. Biol. Chem. 270:10236-10245; ; Park J.-H., et al. (2005) Cancer Res. 65:2804-2814

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 0.2M NaCl, 50% glycerol, 5mM DTT

Cardiovascular

NP_055206

Q15327

Human

MVLKVE ELVTGKKNGN GEAGEFLPED FRDGEYEAAV TLEKQEDLKT LLAHPVTLGE QQWKSEKQRE AELKKKKLEQ RSKLENLEDL EIIIQLKKRK KYRKTKVPVV KEPEPEIITE PVDVPTFLKA ALENKLPVVE KFLSDKNNPD VCDEYKRTAL HRACLEGHLA IVEKLMEAGA QIEFRDMLES TAIHWASRGG NLDVLKLLLN KGAKISARDK LLSTALHVAV RTGHYECAEH LIACEADLNA KDREGDTPLH DAVRLNRYKM IRLLIMYGAD LNIKNCAGKT PMDLVLHWQN GTKAIFDSLR ENSYKTSRIA TF