Recombinant humaan ANKRD54 eiwit

ANKRD54, also known as LIAR, contains 4 ANK repeats. This protein plays an important role in regulating intracellular signaling events associated with erythroid terminal differentiation. This protein interacts (via ankyrin repeat region) with LYN (via SH3-domain) in an activation-independent status of LYN. It forms a multiprotein complex with LYN and HCLS1. ANKRD54 also interacts with TSN2, VAV1, DBNL AND LASP1. Recombinant human ANKRD54 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ankyrin repeat domain-containing protein 54, LIAR, Ankyrin repeat domain containing protein 54

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

34.9 kDa (323aa) confirmed by MALDI-TOF

ANKRD54, Ankyrin repeat domain-containing protein 54, LIAR, Ankyrin repeat domain containing protein 54, ATGP2122-10 µg, ATGP2122-20 µg, ATGP2122-50 µg, ATGP2122-100 µg, ATGP2122-250 µg, ATGP2122-500 µg, ATGP2122-1 mg, ATGP2122-10, ATGP2122-20, ATGP2122-50, ATGP2122-100, ATGP2122-250, ATGP2122-500, ATGP2122-1

Gustafsson, M.O., et al. (2012) Mol. Cell. Biol. 32 (13), 2440-2453 ; ; Olsen, J.V., et al. (2006) Cell 127 (3), 635-648

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Signal Transduction

NP_620152

Q6NXT1

Human

MAAAAGD ADDEPRSGHS SSEGECAVAP EPLTDAEGLF SFADFGSALG GGGAGLSGRA SGGAQSPLRY LHVLWQQDAE PRDELRCKIP AGRLRRAARP HRRLGPTGKE VHALKRLRDS ANANDVETVQ QLLEDGADPC AADDKGRTAL HFASCNGNDQ IVQLLLDHGA DPNQRDGLGN TPLHLAACTN HVPVITTLLR GGARVDALDR AGRTPLHLAK SKLNILQEGH AQCLEAVRLE VKQIIHMLRE YLERLGQHEQ RERLDDLCTR LQMTSTKEQV DEVTDLLASF TSLSLQMQSM EKR