Recombinant humaan Anorganisch Pyrofosfatase/PPA1-eiwit

PPA1 (Pyrophosphatase) belongs to the PPase family. This protein is an enzyme that catalyzes the conversion of one molecule of pyrophosphate to two phosphate ions. The hydrolysis of inorganic pyrophosphate (PPi) to two phosphate ions is utilized in many biochemical pathways to render reactions effectively irreversible. Inorganic pyrophosphatase catalyzes this hydrolysis reaction in the early steps of lipid degradation, a prominent example of this phenomenon. By promoting the rapid hydrolysis of pyrophosphate (PPi), Inorganic pyrophosphatase provides the driving force for the activation of fatty acids destined for oxidation. Recombinant human PPA1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Inorganic pyrophosphatase, IOPPP, PP, PP1, SID6-8061

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

35.2 kDa (313aa) confirmed by MALDI-TOF

Inorganic Pyrophosphatase, PPA1, Inorganic pyrophosphatase, IOPPP, PP, PP1, SID6-8061, ATGP1397-10 µg, ATGP1397-20 µg, ATGP1397-50 µg, ATGP1397-100 µg, ATGP1397-250 µg, ATGP1397-500 µg, ATGP1397-1 mg, ATGP1397-10, ATGP1397-20, ATGP1397-50, ATGP1397-100, ATGP1397-250, ATGP1397-500, ATGP1397-1

Carman GM, et al. (2006) Trends Biochem. Sci. 31 (12) : 694-9.; ; usui Y, et al. (2010) J. Dent. Res. 89 (5) : 504-9.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 200 mM NaCl

Metabolism

NP_066952

Q15181

Human

MGSSHHHHHH SSGLVPRGSH MGSHMSGFST EERAAPFSLE YRVFLKNEKG QYISPFHDIP IYADKDVFHM VVEVPRWSNA KMEIATKDPL NPIKQDVKKG KLRYVANLFP YKGYIWNYGA IPQTWEDPGH NDKHTGCCGD NDPIDVCEIG SKVCARGEII GVKVLGILAM IDEGETDWKV IAINVDDPDA ANYNDINDVK RLKPGYLEAT VDWFRRYKVP DGKPENEFAF NAEFKDKDFA IDIIKSTHDH WKALVTKKTN GKGISCMNTT LSESPFKCDP DAARAIVDAL PPPCESACTV PTDVDKWFHH QKN