Recombinant humaan AP1AR-eiwit

Adaptor-related protein complex 1 associated regulatory protein, also known as AP1AR, is necessary for AP-1 dependent transport between the trans-Golgi network and endosomes. This protein regulates the membrane association of AP1G1/Gamma1-adaptin, one of the subunits of the AP-1 adapter complex. The direct interaction with AP1G1/Gamma1-adaptin attenuates the release of the AP-1 complex from membranes. Recombinant human AP1AR protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Adaptor-related protein complex 1 associated regulatory protein, Adaptor-related protein complex 1 associated regulatory protein, 2C18, C4orf16, gamma-BAR, GBAR, PRO0971

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

36.4 kDa (322aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

AP1AR, Adaptor-related protein complex 1 associated regulatory protein, Adaptor-related protein complex 1 associated regulatory protein, 2C18, C4orf16, gamma-BAR, GBAR, PRO0971, ATGP2321-10 µg, ATGP2321-20 µg, ATGP2321-50 µg, ATGP2321-100 µg, ATGP2321-250 µg, ATGP2321-500 µg, ATGP2321-1 mg, ATGP2321-10, ATGP2321-20, ATGP2321-50, ATGP2321-100, ATGP2321-250, ATGP2321-500, ATGP2321-1

Neubrand V.E., et al. (2005) EMBO J. 24:1122-1133; ; Wollscheid B., et al. (2009) Nat. Biotechnol. 27:378-386;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Signal Transduction

NP_061039

Q63HQ0

Human

MGSSHHHHHH SSGLVPRGSH MGNCCWTQCF GLLRKEAGRL QRVGGGGGSK YFRTCSRGEH LTIEFENLVE SDEGESPGSS HRPLTEEEIV DLRERHYDSI AEKQKDLDKK IQKELALQEE KLRLEEEALY AAQREAARAA KQRKLLEQER QRIVQQYHPS NNGEYQSSGP EDDFESCLRN MKSQYEVFRS SRLSSDATVL TPNTESSCDL MTKTKSTSGN DDSTSLDLEW EDEEGMNRML PMRERSKTEE DILRAALKYS NKKTGSNPTS ASDDSNGLEW ENDFVSAEMD DNGNSEYSGF VNPVLELSDS GIRHSDTDQQ TR