Recombinant humaan AP1S2-eiwit

AP1S2, also known as AP-1 complex subunit sigma-2, serves as the small subunit of AP-1 complex 1 and is a member of the adaptin protein family. Adaptor protein complex 1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors. Recombinant human AP1S2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

AP-1 complex subunit sigma-2, DC22, MGC:1902, MRX59, SIGMA1B

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

20.7 kDa (177aa) confirmed by MALDI-TOF

AP1S2, AP-1 complex subunit sigma-2, DC22, MGC:1902, MRX59, SIGMA1B, ATGP1086-10 µg, ATGP1086-20 µg, ATGP1086-50 µg, ATGP1086-100 µg, ATGP1086-250 µg, ATGP1086-500 µg, ATGP1086-1 mg, ATGP1086-10, ATGP1086-20, ATGP1086-50, ATGP1086-100, ATGP1086-250, ATGP1086-500, ATGP1086-1

Tarpey PS. et al. (2006) Am J Hum Genet. 79:1119-24.; ; Takatsu H. et al. (1998) J Biol Chem. 273 :24693-700

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.1M NaCl,2mM DTT

Signal Transduction

NP_003907

P56377

Human

MQFMLLFSRQ GKLRLQKWYV PLSDKEKKKI TRELVQTVLA RKPKMCSFLE WRDLKIVYKR YASLYFCCAI EDQDNELITL EIIHRYVELL DKYFGSVCEL DIIFNFEKAY FILDEFLLGG EVQETSKKNV LKAIEQADLL QEEAETPRSV LEEIGLT