Recombinant humaan AP3S1-eiwit

AP-3 complex subunit sigma-1, also known as AP3S1, is a Part of the AP-3 complex, an adapter-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. Recombinant human AP3S1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

AP-3 complex subunit sigma-1, CLAPS3, Sigma3A

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

23.8 kDa (213aa) confirmed by MALDI-TOF

AP3S1, AP-3 complex subunit sigma-1, CLAPS3, Sigma3A, ATGP1803-10 µg, ATGP1803-20 µg, ATGP1803-50 µg, ATGP1803-100 µg, ATGP1803-250 µg, ATGP1803-500 µg, ATGP1803-1 mg, ATGP1803-10, ATGP1803-20, ATGP1803-50, ATGP1803-100, ATGP1803-250, ATGP1803-500, ATGP1803-1

Burkard T.R., et al. (2011) BMC Syst. Biol. 5:17-17; ; Dell'Angelica E.C., et al. (1997) EMBO J. 16:917-928;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Transportation

NP_001275

Q92572

Human

MGSSHHHHHH SSGLVPRGSH MIKAILIFNN HGKPRLSKFY QPYSEDTQQQ IIRETFHLVS KRDENVCNFL EGGLLIGGSD NKLIYRHYAT LYFVFCVDSS ESELGILDLI QVFVETLDKC FENVCELDLI FHVDKVHNIL AEMVMGGMVL ETNMNEIVTQ IDAQNKLEKS EAGLAGAPAR AVSAVKNMNL PEIPRNINIG DISIKVPNLP SFK