Recombinant humaan APIP-eiwit

APIP, also known as probable methylthioribulose-1-phosphate dehydratase, catalyzes the dehydration of methylthioribulose-1-phosphate (MTRu-1-P) into 2, 3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) . It has an anti-apoptotic function and prevents muscle ischemic damage. This protein inhibits the cytochrome c-dependent and APAF1-mediated cell death. Recombinant human APIP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Probable methylthioribulose-1-phosphate dehydratase, APIP2, CGI-29, CGI29, dJ179L10.2, MMRP19

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

29.7 kDa (266aa) confirmed by MALDI-TOF

APIP, Probable methylthioribulose-1-phosphate dehydratase, APIP2, CGI-29, CGI29, dJ179L10.2, MMRP19, ATGP1614-10 µg, ATGP1614-20 µg, ATGP1614-50 µg, ATGP1614-100 µg, ATGP1614-250 µg, ATGP1614-500 µg, ATGP1614-1 mg, ATGP1614-10, ATGP1614-20, ATGP1614-50, ATGP1614-100, ATGP1614-250, ATGP1614-500, ATGP1614-1

Cho D.-H., et al. (2004) J. Biol. Chem. 279:39942-39950; ; Lai C.-H., et al. (2000) Genome Res. 10:703-713

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT

Apoptosis

NP_057041

Q96GX9

Human

MGSSHHHHHH SSGLVPRGSH MGSHMSGCDA REGDCCSRRC GAQDKEHPRY LIPELCKQFY HLGWVTGTGG GISLKHGDEI YIAPSGVQKE RIQPEDMFVC DINEKDISGP SPSKKLKKSQ CTPLFMNAYT MRGAGAVIHT HSKAAVMATL LFPGREFKIT HQEMIKGIKK CTSGGYYRYD DMLVVPIIEN TPEEKDLKDR MAHAMNEYPD SCAVLVRRHG VYVWGETWEK AKTMCECYDY LFDIAVSMKK VGLDPSQLPV GENGIV