Recombinant humaan Apolipoproteïne A1/APOA1-eiwit

Apolipoprotein A-1, also known as APOA1, is a human protein with a specific role in lipid metabolism. It is the major protein component of high density lipoprotein (HDL) in plasma. This protein promotes cholesterol efflux from tissues to the liver for excretion. And it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. Defects in the ApoA-1 gene are associated with HDL deficiency including Tangier disease. Recombinant human Apolipoprotein A-1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

APOA1, apolipoprotein A-I, Apolipoprotein A1, Apo-AI, ApoA-I

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

30.3 kDa (264aa) confirmed by MALDI-TOF

Apolipoprotein A-I, ApoA1, APOA1, apolipoprotein A-I, Apolipoprotein A1, Apo-AI, ApoA-I, ATGP0363-10 µg, ATGP0363-20 µg, ATGP0363-50 µg, ATGP0363-100 µg, ATGP0363-250 µg, ATGP0363-500 µg, ATGP0363-1 mg, ATGP0363-10, ATGP0363-20, ATGP0363-50, ATGP0363-100, ATGP0363-250, ATGP0363-500, ATGP0363-1

Wang J., et al. (2009) . Int J Biol Sci. 5 (7) :637-46.; ; Khadem-Ansari MH., et al. (2009) . Singapore Med J. 50 (6) :610-3;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing, 10% glycerol

Metabolism

NP_000030

P02647

Human

MDEPPQSPWD RVKDLATVYV DVLKDSGRDY VSQFEGSALG KQLNLKLLDN WDSVTSTFSK LREQLGPVTQ EFWDNLEKET EGLRQEMSKD LEEVKAKVQP YLDDFQKKWQ EEMELYRQKV EPLRAELQEG ARQKLHELQE KLSPLGEEMR DRARAHVDAL RTHLAPYSDE LRQRLAARLE ALKENGGARL AEYHAKATEH LSTLSEKAKP ALEDLRQGLL PVLESFKVSF LSALEEYTKK LNTQ