Recombinant humaan Apolipoproteïne A5/APOA5-eiwit

APOA5 is an apolipoprotein that plays an important role in regulating the plasma triglyceride levels, a major risk factor for coronary artery disease. It is a component of high density lipoprotein and is highly similar to a rat protein that is upregulated in response to liver injury. Mutations in this gene have been associated with hypertriglyceridemia and hyperlipoproteinemia type 5. This gene is located proximal to the apolipoprotein gene cluster on chromosome 11q23. Alternatively spliced transcript variants encoding the same protein have been identified. Recombinant human APOA5 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Apolipoprotein A-V precursor, Apolipoprotein A-V, APOAV, RAP3

E.coli

Human

His-Tag

SDS-PAGE, Denatured

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

41.3 kDa (366aa)

APOA5, Apolipoprotein A-V precursor, Apolipoprotein A-V, APOAV; RAP3, ATGP2476-10 µg, ATGP2476-20 µg, ATGP2476-50 µg, ATGP2476-100 µg, ATGP2476-250 µg, ATGP2476-500 µg, ATGP2476-1 mg, ATGP2476-10, ATGP2476-20, ATGP2476-50, ATGP2476-100, ATGP2476-250, ATGP2476-500, ATGP2476-1

Weinberg R.B., et al. (2003) J. Biol. Chem. 278:34438-34444.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing , 10% glycerol, 0.4M urea

Metabolism

NP_443200

Q6Q788

Human

RKGFWDY FSQTSGDKGR VEQIHQQKMA REPATLKDSL EQDLNNMNKF LEKLRPLSGS EAPRLPQDPV GMRRQLQEEL EEVKARLQPY MAEAHELVGW NLEGLRQQLK PYTMDLMEQV ALRVQELQEQ LRVVGEDTKA QLLGGVDEAW ALLQGLQSRV VHHTGRFKEL FHPYAESLVS GIGRHVQELH RSVAPHAPAS PARLSRCVQV LSRKLTLKAK ALHARIQQNL DQLREELSRA FAGTGTEEGA GPDPQMLSEE VRQRLQAFRQ DTYLQIAAFT RAIDQETEEV QQQLAPPPPG HSAFAPEFQQ TDSGKVLSKL QARLDDLWED ITHSLHDQGH SHLGDP