Recombinant humaan APPBP1/NAE1-eiwit

NAE1 (NEDD8-activating enzyme E1 regulatory subunit) belongs to the ubiquitin-activating E1 family. This protein binds to the beta-amyloid precursor protein. Beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease. NAE1 participates in a novel ubiquitinylation-related pathway involving the ubiquitin-like molecule NEDD8. Also, this protein is required for cell cycle progression through the S/M checkpoint. Recombinant human NAE1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

NEDD8 activating enzyme E1 subunit 1, A-116A10.1, APPBP1, HPP1, ula-1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

62.7 kDa (557aa)

APPBP1, NAE1, NEDD8 activating enzyme E1 subunit 1, A-116A10.1, APPBP1, HPP1, ula-1, ATGP1355-10 µg, ATGP1355-20 µg, ATGP1355-50 µg, ATGP1355-100 µg, ATGP1355-250 µg, ATGP1355-500 µg, ATGP1355-1 mg, ATGP1355-10, ATGP1355-20, ATGP1355-50, ATGP1355-100, ATGP1355-250, ATGP1355-500, ATGP1355-1

Chen Y, et al. (2000) J Biol Chem. 275 (12) :8929-35.; ; Bohnsack R.N., et al. (2003) J. Biol. Chem. 278:26823-26830

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 200 mM NaCl

Ubiquitination

NP_003896

Q13564

Human

MGSSHHHHHH SSGLVPRGSH MGSMAQLGKL LKEQKYDRQL RLWGDHGQEA LESAHVCLIN ATATGTEILK NLVLPGIGSF TIIDGNQVSG EDAGNNFFLQ RSSIGKNRAE AAMEFLQELN SDVSGSFVEE SPENLLDNDP SFFCRFTVVV ATQLPESTSL RLADVLWNSQ IPLLICRTYG LVGYMRIIIK EHPVIESHPD NALEDLRLDK PFPELREHFQ SYDLDHMEKK DHSHTPWIVI IAKYLAQWYS ETNGRIPKTY KEKEDFRDLI RQGILKNENG APEDEENFEE AIKNVNTALN TTQIPSSIED IFNDDRCINI TKQTPSFWIL ARALKEFVAK EGQGNLPVRG TIPDMIADSG KYIKLQNVYR EKAKKDAAAV GNHVAKLLQS IGQAPESISE KELKLLCSNS AFLRVVRCRS LAEEYGLDTI NKDEIISSMD NPDNEIVLYL MLRAVDRFHK QQGRYPGVSN YQVEEDIGKL KSCLTGFLQE YGLSVMVKDD YVHEFCRYGA AEPHTIAAFL GGAAAQEVIK IITKQFVIFN NTYIYSGMSQ TSATFQL