Recombinant humaan APP/protease Nexin II-eiwit

APP, also known as amyloid beta A4 protein, functions as a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy) . Recombinant human APP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Amyloid beta A4 protein, AAA, AD1, PN2, ABPP, APPI, CVAP, ABETA, CTFgamma

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

34.7 kDa (308aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

APP, Protease Nexin II, ATGP1499-10 µg, ATGP1499-20 µg, ATGP1499-50 µg, ATGP1499-100 µg, ATGP1499-250 µg, ATGP1499-500 µg, ATGP1499-1 mg, ATGP1499-10, ATGP1499-20, ATGP1499-50, ATGP1499-100, ATGP1499-250, ATGP1499-500, ATGP1499-1

Kontush A., et al. (2001) Cell. Mol. Neurobiol. 21:299-315; ; Walter M.F., et al. (2006) Biochem. Biophys. Res. Commun. 233:760-764

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Neuroscience

NP_000475.1

P05067

Human

LEVP TDGNAGLLAE PQIAMFCGRL NMHMNVQNGK WDSDPSGTKT CIDTKEGILQ YCQEVYPELQ ITNVVEANQP VTIQNWCKRG RKQCKTHPHF VIPYRCLVGE FVSDALLVPD KCKFLHQERM DVCETHLHWH TVAKETCSEK STNLHDYGML LPCGIDKFRG VEFVCCPLAE ESDNVDSADA EEDDSDVWWG GADTDYADGS EDKVVEVAEE EEVAEVEEEE ADDDEDDEDG DEVEEEAEEP YEEATERTTS IATTTTTTTE SVEEVVRE