Recombinant humaan APRT-eiwit

APRT (adenine phosphoribosyltransferase) is a 180 amino acid protein that localizes to the cytoplasm and belongs to the purine/pyrimidine phosphoribosyltransferase family. Existing as a homodimer, APRT functions to catalyze the formation of inorganic pyrophosphate and AMP from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP), a reaction that is essential for both purine metabolism and AMP biosynthesis. It also produces adenine as a by-product of the polyamine biosynthesis pathway. Recombinant human APRT protein was expressed in E. coli and purified by using conventional chromatography techniques.

Adenine phosphoribosyltransferase, AMP, Adenine phosphoribosyltransferase AMP diphosphorylase, AMP pyrophosphorylase, DKFZp686D13177, MGC125856, MGC125857, MGC129961, Transphosphoribosidase.

E.coli

Human

Non-Tagged

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

19.6 kDa (180aa) confirmed by MALDI-TOF

APRT, Adenine phosphoribosyltransferase, AMP, Adenine phosphoribosyltransferase AMP diphosphorylase, AMP pyrophosphorylase, DKFZp686D13177, MGC125856, MGC125857, MGC129961, Transphosphoribosidase., ATGP0483-10 µg, ATGP0483-20 µg, ATGP0483-50 µg, ATGP0483-100 µg, ATGP0483-250 µg, ATGP0483-500 µg, ATGP0483-1 mg, ATGP0483-10, ATGP0483-20, ATGP0483-50, ATGP0483-100, ATGP0483-250, ATGP0483-500, ATGP0483-1

Baranowska-Bosiacka I., et al. (2009) Toxicology. 259 (1-2) :77-83.; ; Liang L., et al. (2007) Cancer Res. 67 (5) :1910-7.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Metabolism

NP_000476

P07741

Human

MADSELQLVE QRIRSFPDFP TPGVVFRDIS PVLKDPASFR AAIGLLARHL KATHGGRIDY IAGLDSRGFL FGPSLAQELG LGCVLIRKRG KLPGPTLWAS YSLEYGKAEL EIQKDALEPG QRVVVVDDLL ATGGTMNAAC ELLGRLQAEV LECVSLVELT SLKGREKLAP VPFFSLLQYE