Recombinant humaan ARC/NOL3-eiwit

Nucleolar protein 3 (apoptosis repressor with CARD domain), also known as NOL3, is predominantly expressed in muscle and is present in the nucleoplasm and concentrated in nucleoli. NOL3 mRNA and protein levels are induced by neuronal activity, which is necessary to stimulate neuroplasticity, indicating a potential role for NOL3 in activity-dependent changes in dendrite function. NOL3 has been shown to localize to the cytoskeleton of neuronal cells and appears to colocalize with F-Actin, although it may associate with an actin-associated protein rather than directly with F-Actin. Recombinant human NOL3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Nucleolar protein 3, ARC, MYP, NOP, NOP30

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

25 kDa (231aa) confirmed by MALDI-TOF

NOL3, Nucleolar protein 3, ARC, MYP, NOP, NOP30, ATGP1671-10 µg, ATGP1671-20 µg, ATGP1671-50 µg, ATGP1671-100 µg, ATGP1671-250 µg, ATGP1671-500 µg, ATGP1671-1 mg, ATGP1671-10, ATGP1671-20, ATGP1671-50, ATGP1671-100, ATGP1671-250, ATGP1671-500, ATGP1671-1

Lau L F., et al. (1991) Elseveier Science Pub. 257-293; ; Greenberg M E., et al. (1986) Science. 234: 80-83.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol,1mM DTT, 1mM EDTA

Apoptosis

NP_001171987

O60936

Human

MGNAQER PSETIDRERK RLVETLQADS GLLLDALLAR GVLTGPEYEA LDALPDAERR VRRLLLLVQG KGEAACQELL RCAQRTAGAP DPAWDWQHVG PGYRDRSYDP PCPGHWTPEA PGSGTTCPGL PRASDPDEAG GPEGSEAVQS GTPEEPEPEL EAEASKEAEP EPEPEPELEP EAEAEPEPEL EPEPDPEPEP DFEERDESED S