Recombinant humaan ARF3-eiwit

ARF3, also known as ADP-ribosylation factor 3, functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. This protein involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. Recombinant human ARF3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

ADP-ribosylation factor 3

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

22.8 kDa (201aa) confirmed by MALDI-TOF

ARF3, ADP-ribosylation factor 3, ATGP1215-10 µg, ATGP1215-20 µg, ATGP1215-50 µg, ATGP1215-100 µg, ATGP1215-250 µg, ATGP1215-500 µg, ATGP1215-1 mg, ATGP1215-10, ATGP1215-20, ATGP1215-50, ATGP1215-100, ATGP1215-250, ATGP1215-500, ATGP1215-1

Bobak D.A., et al. (1989) Proc. Natl. Acad. Sci. u.S.A. 86:6101-6105; ; Takeya R., et al. (2000) Biochem. Biophys. Res. Commun. 267:149-155

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT

Signal Transduction

NP_001650

P61204

Human

MGSSHHHHHH SSGLVPRGSH MGNIFGNLLK SLIGKKEMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG FNVETVEYKN ISFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV NEAREELMRM LAEDELRDAV LLVFANKQDL PNAMNAAEIT DKLGLHSLRH RNWYIQATCA TSGDGLYEGL DWLANQLKNK K