Recombinant humaan ARF4-eiwit

ARF4 is a member of the ARF gene family whose members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The ARF proteins include 5 ARF proteins and 11 ARF-like proteins and constitute one family of the RAS superfamily. They are categorized as class I, class II and class III; this gene is a class II member. Recombinant human ARF4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

ADP-ribosylation factor 4, ARF2

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

23.0 kDa (204aa) confirmed by MALDI-TOF

ARF4, ATGP0864-10 µg, ATGP0864-20 µg, ATGP0864-50 µg, ATGP0864-100 µg, ATGP0864-250 µg, ATGP0864-500 µg, ATGP0864-1 mg, ATGP0864-10, ATGP0864-20, ATGP0864-50, ATGP0864-100, ATGP0864-250, ATGP0864-500, ATGP0864-1

Mazelova J., et al. (2009) EMBO J. 28 (3) :183-92.; ; Woo IS., et al. (2009) Cancer Lett. 276 (1) :53-60.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M Nacl, 5mM DTT, 20% glycerol

Signal Transduction

NP_001651

P18085

Human

MGLTIS SLFSRLFGKK QMRILMVGLD AAGKTTILYK LKLGEIVTTI PTIGFNVETV EYKNICFTVW DVGGQDRIRP LWKHYFQNTQ GLIFVVDSND RERIQEVADE LQKMLLVDEL RDAVLLLFAN KQDLPNAMAI SEMTDKLGLQ SLRNRTWYVQ ATCATQGTGL YEGLDWLSNE LSKR