Recombinant humaan ARF4L/ARL4D-eiwit

ARL4D (ADP-ribosylation factor-like protein 4D) is a developmentally regulated member of the ADP-ribosylation factor/ARF-like protein (ARF/ARL) family of Ras-related GTPases. Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP) . This protein may play a role in membrane-associated intracellular trafficking. Mutations in this gene have been associated with Bardet-Biedl syndrome (BBS) . Recombinant human ARL4D protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

ADP-ribosylation factor-like protein 4D, ARF4L, ADP ribosylation factor like GTPase 4D

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

24.3 kDa (221aa) confirmed by MALDI-TOF

ARL4D, ARF4L, ADP-ribosylation factor-like protein 4D, ADP ribosylation factor like GTPase 4D, ATGP1252-10 µg, ATGP1252-20 µg, ATGP1252-50 µg, ATGP1252-100 µg, ATGP1252-250 µg, ATGP1252-500 µg, ATGP1252-1 mg, ATGP1252-010, ATGP1252-020, ATGP1252-050, ATGP1252-100, ATGP1252-250, ATGP1252-500, ATGP1252-01M

Li CC, et al. (2007) Mol Biol Cell. 18 (11) :4420-37.; ; Hofmann I., et al (2007) Curr. Biol. 17:711-716

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 200 mM NaCl

Signal Transduction

NP_001652.2

P49703

Human

MGNHLTEMAP TASSFLPHFQ ALHVVVIGLD SAGKTSLLYR LKFKEFVQSV PTKGFNTEKI RVPLGGSRGI TFQVWDVGGQ EKLRPLWRSY TRRTDGLVFV VDAAEAERLE EAKVELHRIS RASDNQGVPV LVLANKQDQP GALSAAEVEK RLAVRELAAA TLTHVQGCSA VDGLGLQQGL ERLYEMILKR KKAARGGKKR R