Recombinant humaan ARFIP2-eiwit

Arfaptin 2, also known as ARFIP2, is a Rac1 binding protein necessary for Rac-mediated actin polymerization and the subsequent formation of membrane ruffles and lamellipodia. ARFIP2 has also been shown to interact with the ADP ribosylation factor ARF6, a GTPase that associates with the plasma membrane and intracellular endosome vesicles, in a GTP dependent manner. Arfaptin 2 also regulates the aggregation of mutant Huntingtin protein by possibly impairing proteasome function. Expression of ARFIP2 was shown to be increased at sites of neurodegeneration. Recombinant human ARFIP2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Arfaptin 2, POR1

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

40.2 kDa (364aa) confirmed by MALDI-TOF

ARFIP2, Arfaptin 2, POR1, ATGP1695-10 µg, ATGP1695-20 µg, ATGP1695-50 µg, ATGP1695-100 µg, ATGP1695-250 µg, ATGP1695-500 µg, ATGP1695-1 mg, ATGP1695-10, ATGP1695-20, ATGP1695-50, ATGP1695-100, ATGP1695-250, ATGP1695-500, ATGP1695-1

D'Souza Schorey C., et al. (1997) EMBO J. 16: 5445-5454; ; Joneson T., et al. (1996) Science. 274: 1374-1376.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 1mM DTT

Signal Transduction

NP_036534

P53365

Human

MGSSHHHHHH SSGLVPRGSH MGSMTDGILG KAATMEIPIH GNGEARQLPE DDGLEQDLQQ VMVSGPNLNE TSIVSGGYGG SGDGLIPTGS GRHPSHSTTP SGPGDEVARG IAGEKFDIVK KWGINTYKCT KQLLSERFGR GSRTVDLELE LQIELLRETK RKYESVLQLG RALTAHLYSL LQTQHALGDA FADLSQKSPE LQEEFGYNAE TQKLLCKNGE TLLGAVNFFV SSINTLVTKT MEDTLMTVKQ YEAARLEYDA YRTDLEELSL GPRDAGTRGR LESAQATFQA HRDKYEKLRG DVAIKLKFLE ENKIKVMHKQ LLLFHNAVSA YFAGNQKQLE QTLQQFNIKL RPPGAEKPSW LEEQ