Recombinant humaan ARFL1/ARL1-eiwit

ADP-ribosylation factor-like protein 1 (ARL1) belongs to the ARL (ADP-ribosylation factor-like) family of proteins, which are structurally related to ADP-ribosylation factors (ARFs) . ARFs, described as activators of cholera toxin (CT) ADP-ribosyltransferase activity, regulate intracellular vesicular membrane trafficking, and stimulate a phospholipase D (PLD) isoform. Although, ARL proteins were initially thought not to activate CT or PLD, later work showed that they are weak stimulators of PLD and CT in a phospholipid dependent manner. Recombinant human ARL1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

ARFL1, ADP-ribosylation factor-like protein 1, ADP ribosylation factor like GTPase 1

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

22.5 kDa (201aa) confirmed by MALDI-TOF

ARL1, ADP-ribosylation factor-like protein 1, ARFL1, ADP-ribosylation factor-like protein 1, ATGP0660-10 µg, ATGP0660-20 µg, ATGP0660-50 µg, ATGP0660-100 µg, ATGP0660-250 µg, ATGP0660-500 µg, ATGP0660-1 mg, ATGP0660-10, ATGP0660-20, ATGP0660-50, ATGP0660-100, ATGP0660-250, ATGP0660-500, ATGP0660-1

Nishimoto-Morita K., et al. (2009) J. Biol. Chem 284 (16) :10583-92; ; Van Valkenburgh H., et al. (2001) J. Biol. Chem. 276:22826-22837;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 100 mM NaCl, 2mM DTT

Metabolism

NP_001168

P40616

Human

MGSSHHHHHH SSGLVPRGSH MGGFFSSIFS SLFGTREMRI LILGLDGAGK TTILYRLQVG EVVTTIPTIG FNVETVTYKN LKFQVWDLGG QTSIRPYWRC YYSNTDAVIY VVDSCDRDRI GISKSELVAM LEEEELRKAI LVVFANKQDM EQAMTSSEMA NSLGLPALKD RKWQIFKTSA TKGTGLDEAM EWLVETLKSR Q