Recombinant humaan Arginase 1/ARG1-eiwit

Arginase is a manganese-containing enzyme which catalyzes the hydrolysis of arginine to ornithine and urea. It is the final enzyme of the urea cycle. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform functions in the urea cycle, and is located primarily in the cytoplasm of the liver. The type II isoform has been implicated in the regulation of the arginine/ornithine concentrations in the cell. It is located in mitochondria of several tissues in the body, with most abundance in the kidney and prostate. Recombinant human ARG1, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 150,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of arginine to urea per minute at pH 10.5 at 37C.

Arginase-1 liver, Arginase-1, liver, Arginase-1, liver A I, Al, ARG 1, ARG1, Arginase 1, Arginase liver, Arginase type I, Arginase1, Liver type arginase, Type I arginase.

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

Specific activity is > 150,000pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0pmole of arginine to urea per minute at pH 10.5 at 37C.

35.8 kDa (330aa) confirmed by MALDI-TOF

Arginase1, ARG1, ATGP3922-10 µg, ATGP3922-20 µg, ATGP3922-50 µg, ATGP3922-100 µg, ATGP3922-250 µg, ATGP3922-500 µg, ATGP3922-1 mg, ATGP3922-10, ATGP3922-20, ATGP3922-50, ATGP3922-100, ATGP3922-250, ATGP3922-500, ATGP3922-1

Wu G., et al (1998) The Biochemical journal 336 (Pt 1) : 1-17.; ; Iyer RK., et al (2002) Molecular and cellular biology 22 (13) : 4491-8.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 2mM DTT, 100 mM NaCl

Metabolism

NP_000036

P05089

Human