Recombinant humaan Arginase 2/ARG2-eiwit

Arginase is a manganese-containing enzyme which catalyzes the hydrolysis of arginine to ornithine and urea. It is the final enzyme of the urea cycle. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic cross-reactivity and physiologic function. The type II isoform is located in the mitochondria and expressed in extra-hepatic tissues, especially kidney. It is thought to play a role in nitric oxide and polyamine metabolism. Recombinant human ARG2, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Arginase-2 type II, Kidney arginase, Non-hepatic arginase, Arginase-2, type II Arginase II mitochondrial, Kidney type arginase, Non hepatic arginase.

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

38.3 kDa (353aa) confirmed by MALDI-TOF

Arginase2, ARG2, Arginase-2 type II, Kidney arginase, Non-hepatic arginase, Arginase-2, type II Arginase II mitochondrial, Kidney type arginase, Non hepatic arginase., ATGP0591-10 µg, ATGP0591-20 µg, ATGP0591-50 µg, ATGP0591-100 µg, ATGP0591-250 µg, ATGP0591-500 µg, ATGP0591-1 mg, ATGP0591-10, ATGP0591-20, ATGP0591-50, ATGP0591-100, ATGP0591-250, ATGP0591-500, ATGP0591-1

Frumento G., et al. (2008) Int J Cancer. 123 (5) :1108-16J; ; Meurs H., et al. (2010) Pharmacogenet Genomics. 20 (3) :179-86.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Metabolism

NP_001163

P78540

Human

MGSSHHHHHH SSGLVPRGSH MVHSVAVIGA PFSQGQKRKG VEHGPAAIRE AGLMKRLSSL GCHLKDFGDL SFTPVPKDDL YNNLIVNPRS VGLANQELAE VVSRAVSDGY SCVTLGGDHS LAIGTISGHA RHCPDLCVVW VDAHADINTP LTTSSGNLHG QPVSFLLREL QDKVPQLPGF SWIKPCISSA SIVYIGLRDV DPPEHFILKN YDIQYFSMRD IDRLGIQKVM ERTFDLLIGK RQRPIHLSFD IDAFDPTLAP ATFTPVVGGL TYREGMYIAE EIHNTGLLSA LDLVEVNPQL ATSEEEAKTT ANLAVDVIAS SFGQTREGGH IVYDQLPTPS SPDESENQAR VRI