Recombinant humaan ARH/LDLRAP1-eiwit

LDLRAP1 (Low density lipoprotein receptor adapter protein 1) is a cytosolic protein which contains a phosphotyrosine binding (PTD) domain. LDLRAP1 is a cytosolic adaptor that couples LDLR to endocytic machinery. Defects in LDLRAP1 are the cause of autosomal recessive hypercholesterolemia (ARH) . ARH is a disorder caused by defective internalization of LDL receptors (LDLR) in the liver. Recombinant human LDLRAP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Low density lipoprotein receptor adaptor protein 1, ARH, ARH1, ARH2, FHCB1, FHCB2

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

36.1 kDa (328aa) confirmed by MALDI-TOF

LDLRAP1, Low density lipoprotein receptor adaptor protein 1, ARH, ARH1, ARH2, FHCB1, FHCB2, ATGP1396-10 µg, ATGP1396-20 µg, ATGP1396-50 µg, ATGP1396-100 µg, ATGP1396-250 µg, ATGP1396-500 µg, ATGP1396-1 mg, ATGP1396-10, ATGP1396-20, ATGP1396-50, ATGP1396-100, ATGP1396-250, ATGP1396-500, ATGP1396-1

Garcia C.K., et al. (2001) Science 292:1394-1398; ; Mishra S.K., et al. (2005) J. Biol. Chem. 280:19270-19280

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 200 mM NaCl

Metabolism

AAH29770

Q5SW96

Human

MGSSHHHHHH SSGLVPRGSH MDALKSAGRA LIRSPSLAKQ SWGGGGRHRK LPENWTDTRE TLLEGMLFSL KYLGMTLVEQ PKGEELSAAA IKRIVATAKA SGKKLQKVTL KVSPRGIILT DNLTNQLIEN VSIYRISYCT ADKMHDKVFA YIAQSQHNQS LECHAFLCTK RKMAQAVTLT VAQAFKVAFE FWQVSKEEKE KRDKASQEGG DVLGARQDCT PPLKSLVATG NLLDLEETAK APLSTVSANT TNMDEVPRPQ ALSGSSVVWE LDDGLDEAFS RLAQSRTNPQ VLDTGLTAQD MHYAQCLSPV DWDKPDSSGT EQDDLFSF